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  • FGF1 Antibodies

Invitrogen

FGF1 Polyclonal Antibody, PeproTech®

2 References
View all (19) FGF1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite FGF1 Polyclonal Antibody, PeproTech®

  • Antibody Testing Data (3)
FGF1 Antibody in Western Blot (WB)
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FGF1 Antibody in Western Blot (WB)
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FGF1 Antibody (500-P17-1MG) in WB

Western Blot: To detect Human FGF-acidic by Western Blot analysis FGF1 Polyclonal Antibody (Product # 500-P17-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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FGF1 Antibody in Western Blot (WB)
FGF1 Antibody in Western Blot (WB)
FGF1 Antibody in ELISA (ELISA)
FGF1 Polyclonal Antibody, PeproTech®

Product Details

500-P17-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human FGF-acidic
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929622

Product Specific Information

AA Sequence of recombinant protein: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-acidic. Anti-Human FGF-acidic-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-acidic matrix.

Sandwich ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-acidic (500-P17Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-acidic.

Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; ECGF; Endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; FGF; FGF-1; Fibroblast growth factor; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); H-FGF-a; HBGF-1; Heparin-binding growth factor 1; M-FGF-a

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Gene Aliases: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGF1; FGFA; GLIO703; HBGF-1; HBGF1

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UniProt ID: (Human) P05230

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Entrez Gene ID: (Human) 2246

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Function(s)
protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding integrin binding protein binding growth factor activity heparin binding 1-phosphatidylinositol-3-kinase activity Hsp70 protein binding S100 protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity
Process(es)
MAPK cascade activation of MAPK activity angiogenesis organ induction signal transduction multicellular organism development cell proliferation positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway anatomical structure morphogenesis positive regulation of endothelial cell migration regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cell differentiation lung development positive regulation of cell migration activation of protein kinase B activity cellular response to heat phosphatidylinositol-3-phosphate biosynthetic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of cholesterol biosynthetic process positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of epithelial cell proliferation positive regulation of cell division branch elongation involved in ureteric bud branching positive regulation of ERK1 and ERK2 cascade mesonephric epithelium development regulation of endothelial tube morphogenesis positive regulation of intracellular signal transduction positive regulation of sprouting angiogenesis regulation of endothelial cell chemotaxis to fibroblast growth factor
It has to be done as per old AB suggested Products section.
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