Invitrogen

FADS1 Recombinant Rabbit Monoclonal Antibody (JE55-63)

Name: FADS1 Recombinant Rabbit Monoclonal Antibody (JE55-63)
Brand: Invitrogen
SKU: MA5-42500

View all (14) FADS1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)

FADS1 Antibody in Immunocytochemistry (ICC/IF)

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FADS1 Antibody (MA5-42500) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of FADS1 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with FADS1 Recombinant Monoclonal Antibody (Product # MA5-42500) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue)... View More

FADS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image FADS1 Recombinant Rabbit Monoclonal Antibody (JE55-63)

Product Details

MA5-42500

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:100

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE55-63

Immunogen

Synthetic peptide within N-terminal Human FADS1

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2911641

Product Specific Information

Positive Control: Rat liver tissue lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat lung tissue lysate, Hela cell lysate, A549 cell lysate, Hela, PANC-1, human liver carcinoma tissue, human thyroid tissue, human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, A549.

Subcellular Location: Mitochondrion, Endoplasmic reticulum membrane.

Target Information

The protein encoded by this gene is a member of the fatty acid desaturase gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13. 1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acyl-CoA (8-3)-desaturase; BC269730_2; D5D; delta(5) desaturase; Delta(5) fatty acid desaturase; delta-5 desaturase; delta-5 fatty acid desaturase; Fatty acid desaturase 1; FLJ38956; FLJ90273; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1

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Gene Aliases: 0710001O03Rik; A930006B21Rik; AI317215; D5D; DSD; FADS1; FADS6; FADSD5; LLCDL1; TU12

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UniProt ID: (Human) O60427, (Mouse) Q920L1, (Rat) Q920R3

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Entrez Gene ID: (Human) 3992, (Mouse) 76267, (Rat) 84575

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Function(s)

C-5 sterol desaturase activity oxidoreductase activity omega-6 fatty acid desaturase activity linoleoyl-CoA desaturase activity acyl-CoA desaturase activity oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water iron ion binding heme binding

Process(es)

regulation of transcription, DNA-templated unsaturated fatty acid biosynthetic process cell-cell signaling phospholipid biosynthetic process cellular response to starvation alpha-linolenic acid metabolic process linoleic acid metabolic process regulation of cell differentiation icosanoid biosynthetic process oxidation-reduction process lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process transport arachidonic acid metabolic process long-chain fatty acid biosynthetic process aging response to nutrient response to sucrose response to organic substance response to organic cyclic compound response to insulin response to vitamin A response to isolation stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FADS1 Recombinant Rabbit Monoclonal Antibody (JE55-63)

FADS1 Antibody (MA5-42500) in ICC/IF

Product Details

MA5-42500

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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