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  • Primary Antibodies ›
  • Endomucin Antibodies

Invitrogen

Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), Alexa Fluor™ 488, eBioscience™

20 Published Figures
16 References
View all (15) Endomucin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), Alexa Fluor™ 488, eBioscience™

  • Antibody Testing Data (1)
  • Published Figures (20)
Endomucin Antibody in Flow Cytometry (Flow)
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Endomucin Antibody in Flow Cytometry (Flow)
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Endomucin Antibody (53-5851-82) in Flow

bEnd3 cells were stained with 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (blue histogram) or 0.5 µg of Endomucin Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Endomucin Antibody in Flow Cytometry (Flow)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunocytochemistry (ICC/IF)
Endomucin Antibody in Immunocytochemistry (ICC/IF)
Endomucin Antibody in Immunocytochemistry (ICC/IF)
Endomucin Antibody in Immunocytochemistry (ICC/IF)
Endomucin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Immunohistochemistry (IHC)
Endomucin Antibody in Flow Cytometry (Flow)
Endomucin Antibody in Flow Cytometry (Flow)
Endomucin Antibody in Flow Cytometry (Flow)
Endomucin Antibody in Flow Cytometry (Flow)

Product Details

53-5851-82

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications

Flow Cytometry (Flow)

1.0 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioV.7C7 (V.7C7)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
  • Unconjugated
  • eFluor 660
  • PE 
  • Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802332

Product Specific Information

Description: The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, which was identified in a search for cell-surface expressed endothelial cell markers. Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however, an exception is the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.

Applications Reported: This eBioV.7C7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioV.7C7 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

Endomucin (EMCN) also known as endothelial sialomucin or mucin-like sialoglycoprotein interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: Endomucin; endomucin-1; Endomucin-1/2; MUC-14; mucin 14, endothelial; Mucin-14

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Gene Aliases: 0610012K22Rik; AI315669; Emcn; Muc14

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UniProt ID: (Mouse) Q9R0H2

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Entrez Gene ID: (Mouse) 59308

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It has to be done as per old AB suggested Products section.
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