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  • ERK1/2 Antibodies

Invitrogen

ERK1/ERK2 Monoclonal Antibody (K.913.4)

Advanced Verification
4 Published Figures
9 References
View all (85) ERK1/2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite ERK1/ERK2 Monoclonal Antibody (K.913.4)

  • Antibody Testing Data (7)
  • Published Figures (4)
  • Advanced Verification (3)
ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
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ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
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ERK1/ERK2 Antibody (MA5-15134) in ICC/IF

Immunofluorescence analysis of ERK1/ERK2 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK1/ERK2 Rabbit Monoclonal Antibody(K.913.4) (Product # MA5-15134) at 1:100 dilution in 0.1% BSA, incubated at ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
ERK1/ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
ERK1/ERK2 Antibody in Western Blot (WB)
ERK1/ERK2 Antibody in Western Blot (WB)
ERK1/ERK2 Antibody in Flow Cytometry (Flow)
ERK1/ERK2 Antibody in Immunocytochemistry (ICC/IF)
ERK1/ERK2 Antibody in Western Blot (WB)
ERK1/ERK2 Antibody in Western Blot (WB)
ERK1/ERK2 Antibody in Western Blot (WB)
ERK1/ERK2 Antibody
ERK1/ERK2 Antibody
ERK1/ERK2 Antibody
ERK1/ERK2 Monoclonal Antibody (K.913.4)

Product Details

MA5-15134

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:125-1:500
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
View 2 publications 2 publications

Flow Cytometry (Flow)

1:200-1:800
-

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Bovine, Dog, C. elegans, Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish

Published species

Chicken, Human, Pig, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.913.4

Immunogen

A synthetic peptide corresponding to residues near the C-terminus of human p44 MAP kinase.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

84 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982335

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with JNK/SAPK or p38 MAP kinase.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; Diphospho-ERK; drosophila ERK; enhancer of seven in absentia 7; ERK; Erk MAP kinase; ERK-1; ERK-2; ERK/rolled; erk1 erk2; ERK1b; ERK2; ERT1; ERT2; extracellular signal-regulated kinase; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; Insulin-stimulated MAP2 kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAP-kinase; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen-activated 3; mitogen-activated protein kinase; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; phospho-ERK; pp42/MAP kinase; protein kinase; Protein rolled; protein tyrosine kinase ERK2; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sevenmaker

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Gene Aliases: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; BOS_16809; BOS_22892; C78273; CG12559; CG18732; CT34260; CT39192; D-ERK; DERK; DERK-A; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERK-A; DmERKA; DmMAPK; dp-ERK; DpERK; dpERK1; dpMAPK; Dsor2; E(sina)7; EC2-1; EK2-1; ERK; ERK-1; ERK-2; ERK-A; Erk/Map kinase; ERK1; ERK2; ERKA; ERT1; ERT2; Esrk1; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; l(2)41Ac; l(2R)EMS45-39; MAP-k; MAPK; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; pERK; pMAPK; PRKM1; PRKM2; PRKM3; RL; rl/ERK; rl/MAPK; rll; SEM; SR2-1; Su(Raf)2B; wu:fi06b09; zERK1; zERK2

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UniProt ID: (Human) P28482, (Bovine) P46196, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708, (Fruit fly) P40417

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Entrez Gene ID: (Human) 5594, (Dog) 477575, (Bovine) 327672, (Pig) 100153927, (Bovine) 531391, (Human) 5595, (Dog) 100855877, (Chinese hamster) 100759514, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Chinese hamster) 100753149, (Zebrafish) 360144, (Zebrafish) 399480, (Fruit fly) 3354888

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Function(s)
phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups JUN kinase activity
Process(es)
MAPK cascade activation of MAPK activity MAPK import into nucleus positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly protein phosphorylation apoptotic process DNA damage induced protein phosphorylation cell cycle axon guidance fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process platelet activation BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter thymus development regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding eye development brain development regulation of gene expression central nervous system projection neuron axonogenesis intracellular signal transduction chordate embryonic development cellular response to organic substance transcription, DNA-templated chemotaxis response to stress cellular response to DNA damage stimulus signal transduction chemical synaptic transmission positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process positive regulation of cell migration regulation of protein stability mammary gland epithelial cell proliferation ERBB signaling pathway response to estrogen negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to granulocyte macrophage colony-stimulating factor stimulus organ morphogenesis response to lipopolysaccharide regulation of transcription, DNA-templated mitotic nuclear division transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway gastrulation imaginal disc-derived wing vein specification imaginal disc-derived wing morphogenesis heart development metamorphosis torso signaling pathway determination of adult lifespan imaginal disc-derived wing vein morphogenesis anterior/posterior axis specification, embryo cellular response to starvation negative regulation of macroautophagy adherens junction maintenance cellular response to reactive oxygen species negative regulation of apoptotic process R7 cell development sevenless signaling pathway positive regulation of photoreceptor cell differentiation positive regulation of Ras protein signal transduction behavioral response to ethanol regulation of synapse structure or activity modulation of synaptic transmission cellular response to arsenic-containing substance cellular response to cadmium ion positive regulation of wound healing regulation of heart morphogenesis regulation of response to drug
It has to be done as per old AB suggested Products section.
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