Invitrogen

ERK1/ERK2 Monoclonal Antibody (E.657.8)

Advanced Verification
View all (85) ERK1/2 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15227

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-
Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

E.657.8

Immunogen

Synthetic peptide corresponding to the sequence of p44/42 MAP kinase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

75 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980663

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with either JNK/SAPK or p38 MAP kinase.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERK2; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein kinase; protein tyrosine kinase ERK2

View more View less

Gene Aliases: 9030612K14Rik; AA407128; AU018647; BOS_16809; BOS_22892; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; fi06b09; HS44KDAP; HUMKER1A; I79_009500; I79_018350; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3; wu:fi06b09; zERK1; zERK2

View more View less

UniProt ID: (Human) P28482, (Bovine) P46196, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708

View more View less

Entrez Gene ID: (Human) 5594, (Bovine) 327672, (Pig) 100153927, (Bovine) 531391, (Human) 5595, (Chinese hamster) 100759514, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Chinese hamster) 100753149, (Zebrafish) 360144, (Zebrafish) 399480

View more View less

Function(s)
phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding protein kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
MAPK cascade activation of MAPK activity MAPK import into nucleus positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly protein phosphorylation apoptotic process DNA damage induced protein phosphorylation cell cycle axon guidance fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process platelet activation BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter thymus development regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding eye development brain development regulation of gene expression central nervous system projection neuron axonogenesis intracellular signal transduction chordate embryonic development cellular response to organic substance transcription, DNA-templated chemotaxis response to stress cellular response to DNA damage stimulus signal transduction chemical synaptic transmission positive regulation of cell proliferation positive regulation of peptidyl-threonine phosphorylation peptidyl-threonine phosphorylation cytosine metabolic process positive regulation of cell migration regulation of protein stability mammary gland epithelial cell proliferation ERBB signaling pathway response to estrogen negative regulation of cell differentiation positive regulation of transcription, DNA-templated T cell receptor signaling pathway B cell receptor signaling pathway long-term synaptic potentiation labyrinthine layer blood vessel development cellular response to granulocyte macrophage colony-stimulating factor stimulus organ morphogenesis response to lipopolysaccharide gastrulation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support