NeoBiotechnologies

ER-beta (Estrogen Receptor beta-1) Monoclonal Antibody (PGP5/10)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
ER-beta (Estrogen Receptor beta-1) Monoclonal Antibody (PGP5/10)

Product Details

2100-MSM10-P1ABX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2-4 µg/mL
-

Flow Cytometry (Flow)

2-4 µg/10^6 cells
-
Product Specifications

Species Reactivity

Horse, Human, Mouse, Non-human primate, Sheep, Pig, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

PGP5/10

Immunogen

A Synthetic peptide (CEDSKSKEGSQNPQSQ) from the C terminus of the human estrogen receptor beta 1 isomer
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ER; ER beta; ER-beta; estrogen receptor 2 (ER beta); estrogen receptor 2 beta; Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; steroid receptor

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Gene Aliases: er; ER-BETA; ER[b]; ERB; Erb2; ERBETA; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2

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UniProt ID: (Pig) Q9XSW2, (Human) Q92731, (Sheep) Q9TU15, (Mouse) O08537, (Rat) Q62986

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Entrez Gene ID: (Pig) 396697, (Human) 2100, (Horse) 100033964, (Sheep) 443066, (Mouse) 13983, (Rat) 25149

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Function(s)
core promoter sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding drug binding zinc ion binding enzyme binding estrogen receptor activity estrogen response element binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding peroxisome proliferator activated receptor binding receptor antagonist activity estradiol binding steroid hormone binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding lipid binding hormone binding sequence-specific DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development behavioral fear response neuron migration transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter brain development learning or memory negative regulation of cell proliferation hormone-mediated apoptotic signaling pathway intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to testosterone regulation of cell proliferation positive regulation of apoptotic process steroid hormone mediated signaling pathway regulation of neuron apoptotic process estrous cycle positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of behavior negative regulation of smooth muscle cell proliferation negative regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell proliferation uterus development vagina development negative regulation of cell death prostate gland epithelium morphogenesis epithelial cell maturation involved in prostate gland development negative regulation of androgen receptor signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of neuron death negative regulation of feeding behavior negative regulation of reactive oxygen species metabolic process transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling response to water deprivation response to activity response to insecticide cerebellum development amygdala development hypothalamus development negative regulation of cell growth prostate gland development response to nutrient levels response to genistein cellular response to drug response to estrogen response to human chorionic gonadotropin response to ethanol positive regulation of vasodilation Sertoli cell development cellular response to magnetism cellular response to estradiol stimulus response to dexamethasone extracellular negative regulation of signal transduction response to salt response to bisphenol A male gonad development female gonad development response to hormone response to organic cyclic compound response to estradiol response to drug cellular response to organic cyclic compound
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