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  • eIF4A3 Antibodies

Proteintech

EIF4A3 Monoclonal Antibody (1G6H9)

View all (16) eIF4A3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite EIF4A3 Monoclonal Antibody (1G6H9)

  • Antibody Testing Data (2)
EIF4A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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EIF4A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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EIF4A3 Antibody (67740-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EIF4A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
EIF4A3 Antibody in Western Blot (WB)

Product Details

67740-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:25,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:2,500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1G6H9

Immunogen

EIF4A3 Fusion Protein Ag11130

Conjugate

Unconjugated Unconjugated Unconjugated
  • CoraLite Plus 488
  • CoraLite Plus 647
  • Request custom conjugation

Form

Liquid

Concentration

500 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

eIF4A3 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-III; eIF4A-III; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 3; hNMP 265; NMP 265; Nuclear matrix protein 265

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Gene Aliases: 2400003O03Rik; DDX48; eIF4A-III; EIF4A3; eIF4AIII; KIAA0111; mKIAA0111; MUK34; NMP265; NUK34; RCPS

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UniProt ID: (Human) P38919, (Mouse) Q91VC3, (Rat) Q3B8Q2

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Entrez Gene ID: (Human) 9775, (Mouse) 192170, (Rat) 688288

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Function(s)
mRNA binding ATP-dependent RNA helicase activity protein binding ATP binding poly(A) binding selenocysteine insertion sequence binding RNA stem-loop binding ribonucleoprotein complex binding poly(A) RNA binding
Process(es)
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening mRNA splicing, via spliceosome rRNA processing termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus associative learning RNA splicing RNA secondary structure unwinding response to organic cyclic compound selenocysteine metabolic process negative regulation of translation mRNA 3'-end processing exploration behavior positive regulation of translation embryonic cranial skeleton morphogenesis cellular response to selenite ion negative regulation of excitatory postsynaptic potential negative regulation of selenocysteine incorporation negative regulation of selenocysteine insertion sequence binding cellular response to brain-derived neurotrophic factor stimulus positive regulation of transcription from RNA polymerase II promoter positive regulation of mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome mRNA processing mRNA transport
It has to be done as per old AB suggested Products section.
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