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  • EEF2 Antibodies

Invitrogen

EEF2 Recombinant Rabbit Monoclonal Antibody (4E2Z5)

View all (31) EEF2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite EEF2 Recombinant Rabbit Monoclonal Antibody (4E2Z5)

  • Antibody Testing Data (6)
EEF2 Antibody in Immunocytochemistry (ICC/IF)
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EEF2 Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 6

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EEF2 Antibody (MA5-42937) in ICC/IF

Immunofluorescence analysis of EEF2 in NIH-3T3 cells. Samples were incubated with EEF2 Monoclonal antibody (Product # MA5-42937) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EEF2 Antibody in Immunocytochemistry (ICC/IF)
EEF2 Antibody in Immunocytochemistry (ICC/IF)
EEF2 Antibody in Immunocytochemistry (ICC/IF)
EEF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
EEF2 Antibody in Western Blot (WB)
EEF2 Antibody in Western Blot (WB)
EEF2 Recombinant Rabbit Monoclonal Antibody (4E2Z5)

Product Details

MA5-42937

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:6,000
-

Immunohistochemistry (IHC)

-
- -

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4E2Z5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF2 (P13639).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912078

Product Specific Information

Positive test controls include: HeLa, NIH/3T3, Jurkat, Mouse liver, Rat spleen. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: IYGVLNRKRG HVFEESQVAG TPMFVVKAYL PVNESFGFTA DLRSNTGGQA FPQCVFDHWQ ILPGDPFDNS SRPSQVVAET RKRKGLKEGI PALDNFLDKL

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EF-2; Elongation factor 2; elongation factor-2; polypeptidyl-tRNA translocase

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Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26

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UniProt ID: (Human) P13639, (Mouse) P58252, (Rat) P05197

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Entrez Gene ID: (Human) 1938, (Mouse) 13629, (Rat) 29565

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Function(s)
p53 binding translation elongation factor activity GTPase activity protein binding GTP binding 5S rRNA binding protein kinase binding ribosome binding poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding translation activator activity
Process(es)
hematopoietic progenitor cell differentiation translation translational elongation positive regulation of translation response to ischemia skeletal muscle contraction aging glial cell proliferation response to fluoxetine peptidyl-diphthamide biosynthetic process from peptidyl-histidine response to estradiol response to endoplasmic reticulum stress skeletal muscle cell differentiation response to hydrogen peroxide response to ethanol response to folic acid cell-cell adhesion cellular response to brain-derived neurotrophic factor stimulus positive regulation of cytoplasmic translation
It has to be done as per old AB suggested Products section.
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