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  • EEF2 Antibodies

Invitrogen

EEF2 Polyclonal Antibody

View all (31) EEF2 antibodies

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Datasheet
Protocols
Questions & Answers

Cite EEF2 Polyclonal Antibody

  • Antibody Testing Data (1)
EEF2 Antibody in Western Blot (WB)
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EEF2 Antibody in Western Blot (WB)
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EEF2 Antibody (PA5-27661) in WB

Western Blot using EEF2 Polyclonal Antibody (Product # PA5-27661). Sample (30 µg of whole cell lysate). Lane A: MCF-7 . 7.5% SDS PAGE. EEF2 Polyclonal Antibody (Product # PA5-27661) diluted at 1:1,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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EEF2 Antibody in Western Blot (WB)
EEF2 Polyclonal Antibody

Product Details

PA5-27661

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human eEF2. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.94 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2545137

Product Specific Information

Recommended positive controls: MCF-7.

Predicted reactivity: Mouse (99%), Rat (99%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EF-2; Elongation factor 2; polypeptidyl-tRNA translocase

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Gene Aliases: EEF-2; EEF2; EF-2; EF2; SCA26

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UniProt ID: (Human) P13639

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Entrez Gene ID: (Human) 1938

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Function(s)
p53 binding translation elongation factor activity GTPase activity protein binding GTP binding 5S rRNA binding protein kinase binding ribosome binding poly(A) RNA binding actin filament binding cadherin binding involved in cell-cell adhesion
Process(es)
hematopoietic progenitor cell differentiation response to ischemia skeletal muscle contraction translational elongation aging glial cell proliferation response to fluoxetine peptidyl-diphthamide biosynthetic process from peptidyl-histidine response to estradiol response to endoplasmic reticulum stress skeletal muscle cell differentiation response to hydrogen peroxide response to ethanol positive regulation of translation response to folic acid cell-cell adhesion cellular response to brain-derived neurotrophic factor stimulus positive regulation of cytoplasmic translation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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