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  • E2F4 Antibodies

Bethyl Laboratories

E2F4 Polyclonal Antibody

View all (35) E2F4 antibodies

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Cite E2F4 Polyclonal Antibody

  • Antibody Testing Data (3)
E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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E2F4 Antibody (A302-134A) in IHC (P)

Detection of human E2F4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 (Product # A302-134A) (Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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E2F4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
E2F4 Antibody in Western Blot (WB)
E2F4 Antibody in Immunoprecipitation (IP)
E2F4 Polyclonal Antibody

Product Details

A302-134A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 363 and 413 of human E2F transcription factor 4.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; Transcription factor E2F4

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Gene Aliases: E2F-4; E2F4

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UniProt ID: (Human) Q16254

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Entrez Gene ID: (Human) 1874

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding promoter-specific chromatin binding
Process(es)
regulation of transcription involved in G1/S transition of mitotic cell cycle epithelial cell development transcription from RNA polymerase II promoter cell volume homeostasis DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest blood circulation organ morphogenesis regulation of cell proliferation motile cilium assembly positive regulation of transcription from RNA polymerase II promoter centriole assembly multi-ciliated epithelial cell differentiation
It has to be done as per old AB suggested Products section.
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