Fabgennix

E2F1 Polyclonal Antibody, Biotin

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Datasheet
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Datasheet
Protocols
Questions & Answers
E2F1 Polyclonal Antibody, Biotin

Product Details

E2F1M-BIOTIN

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1:10,000
-

Immunoprecipitation (IP)

1:50-1:250
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide taken within amino acid region 125-165 on E2F1 protein.
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5-1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 7.4-7.8, with 30% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1

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Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3

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UniProt ID: (Human) Q01094, (Mouse) Q61501, (Rat) O09139

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Entrez Gene ID: (Human) 1869, (Mouse) 13555, (Rat) 399489

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Function(s)
core promoter binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
DNA damage checkpoint negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process cell cycle intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of gene expression forebrain development anoikis negative regulation of DNA binding negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation mRNA stabilization regulation of cell cycle negative regulation of fat cell proliferation cellular response to xenobiotic stimulus negative regulation of transcription involved in G1/S transition of mitotic cell cycle intrinsic apoptotic signaling pathway by p53 class mediator lens fiber cell apoptotic process cellular response to nerve growth factor stimulus regulation of G1/S transition of mitotic cell cycle G1/S transition of mitotic cell cycle spermatogenesis cellular response to fatty acid cellular response to hypoxia DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
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