Bethyl Laboratories

E2F1 Polyclonal Antibody

Name: E2F1 Polyclonal Antibody
Brand: Bethyl Laboratories
SKU: A300-766A

View all (58) E2F1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)

FIGURE: 1 / 2

E2F1 Antibody (A300-766A) in WB

Detection of human E2F1 by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, HeLa, OVCAR-8, KG-1, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F1 antibody (Product # A300-766A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

E2F1 Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image E2F1 Polyclonal Antibody

Product Details

A300-766A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,500-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 387 and 437 of human E2F Transcription Factor 1.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse

Target Information

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1

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Gene Aliases: E2F-1; E2F1; RBAP1; RBBP3; RBP3

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UniProt ID: (Human) Q01094

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Entrez Gene ID: (Human) 1869

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Function(s)

core promoter binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein kinase binding sequence-specific DNA binding

Process(es)

DNA damage checkpoint negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest spermatogenesis intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of gene expression forebrain development anoikis negative regulation of DNA binding negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation mRNA stabilization negative regulation of fat cell proliferation cellular response to fatty acid cellular response to hypoxia cellular response to xenobiotic stimulus negative regulation of transcription involved in G1/S transition of mitotic cell cycle intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway lens fiber cell apoptotic process cellular response to nerve growth factor stimulus regulation of G1/S transition of mitotic cell cycle

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E2F1 Polyclonal Antibody

E2F1 Antibody (A300-766A) in WB

Product Details

A300-766A

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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