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  • DMC1 Antibodies

Invitrogen

DMC1 Polyclonal Antibody

View all (16) DMC1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DMC1 Polyclonal Antibody

  • Antibody Testing Data (3)
DMC1 Antibody in Immunocytochemistry (ICC/IF)
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DMC1 Antibody in Immunocytochemistry (ICC/IF)
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DMC1 Antibody (PA5-104492) in ICC/IF

Immunocytochemistry analysis of DMC1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with DMC1 polyclonal antibody (Product # PA5-104492) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DMC1 Antibody in Immunocytochemistry (ICC/IF)
DMC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DMC1 Antibody in Western Blot (WB)
DMC1 Polyclonal Antibody

Product Details

PA5-104492

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human DMC1(Accession Q14565), corresponding to amino acid residues E67-E117.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2853793

Product Specific Information

Antibody detects endogenous levels of total DMC1.

Target Information

DMC1 is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this protein is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: disrupted meiotic cDNA1, yeast, homolog of; DMC 1; DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination; DMC1 homologue; HsLim15; Meiotic recombination protein DMC1/LIM15 homolog; MGC150472; MGC150473; RP1-199H16.4

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Gene Aliases: dJ199H16.1; DMC1; DMC1H; LIM15

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UniProt ID: (Human) Q14565

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Entrez Gene ID: (Human) 11144, (Rat) 362960

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Function(s)
recombinase activity four-way junction DNA binding DNA binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding DNA-dependent ATPase activity
Process(es)
ovarian follicle development oocyte maturation DNA repair synapsis reciprocal meiotic recombination male meiosis I gamete generation spermatogenesis spermatid development DNA recombinase assembly mitotic recombination meiotic nuclear division female gamete generation response to ionizing radiation strand invasion chromosome organization involved in meiotic cell cycle
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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