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  • DGCR8 Antibodies

Invitrogen

DGCR8 Polyclonal Antibody

Advanced Verification
1 Published Figure
3 References
View all (21) DGCR8 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DGCR8 Polyclonal Antibody

  • Antibody Testing Data (5)
  • Published Figures (1)
  • Advanced Verification (1)
DGCR8 Antibody in Immunocytochemistry (ICC/IF)
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DGCR8 Antibody in Immunocytochemistry (ICC/IF)
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DGCR8 Antibody (PA5-78510) in ICC/IF

Immunofluorescence analysis of DGCR8 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DGCR8 Polyclonal Antibody (Product # PA5-78510) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DGCR8 Antibody in Immunocytochemistry (ICC/IF)
DGCR8 Antibody in Western Blot (WB)
DGCR8 Antibody in Western Blot (WB)
DGCR8 Antibody in Western Blot (WB)
DGCR8 Antibody in Western Blot (WB)
DGCR8 Antibody in Western Blot (WB)
DGCR8 Antibody
DGCR8 Polyclonal Antibody

Product Details

PA5-78510

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
-

ChIP assay (ChIP)

-
View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735717

Product Specific Information

Positive Control: 293T, A431

Predicted Reactivity: Mouse (95%), Rat (92%), Pig (93%), Rhesus Monkey (99%), Bovine (92%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DiGeorge syndrome critical region 8; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8

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Gene Aliases: C22orf12; D16H22S1742E; D16H22S788E; D16Wis2; DGCR8; DGCRK6; Gy1; LP4941; mir-1306; N41; pasha; Vo59c07

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UniProt ID: (Human) Q8WYQ5, (Mouse) Q9EQM6

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Entrez Gene ID: (Human) 54487, (Mouse) 94223

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Function(s)
double-stranded RNA binding ribonuclease III activity protein binding heme binding protein homodimerization activity metal ion binding primary miRNA binding RNA binding
Process(es)
gene silencing by RNA primary miRNA processing regulation of stem cell proliferation RNA phosphodiester bond hydrolysis, endonucleolytic RNA phosphodiester bond hydrolysis
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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