Invitrogen

DDX3 Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers
DDX3 Polyclonal Antibody

Product Details

PA5-144092

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

1:200-1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human ATP-dependent RNA helicase DDX3X protein (2-662AA)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.565 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093513

Target Information

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX3X; CAP-Rf; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 3; D1Pas1-related sequence 2; DBX; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 3; DEAD box protein 3, X-chromosomal; DEAD box RNA helicase DEAD3; DEAD box, X isoform; DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked; DEAD/H box-3; Embryonic RNA helicase; fibroblast growth factor inducible 14; Helicase-like protein 2; HLP2; mDEAD3

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Gene Aliases: CAP-Rf; D1Pas1-rs2; DBX; DDX14; DDX3; DDX3X; Dead3; Erh; Fin14; HLP2; MRX102

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UniProt ID: (Human) O00571, (Mouse) Q62167

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Entrez Gene ID: (Human) 1654, (Rat) 317335, (Mouse) 13205

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Function(s)
DNA binding RNA binding GTPase activity ATP-dependent DNA helicase activity ATP-dependent RNA helicase activity protein binding ATP binding transcription factor binding poly(A) binding eukaryotic initiation factor 4E binding ATPase activity nucleoside-triphosphatase activity translation initiation factor binding RNA stem-loop binding ribosomal small subunit binding CTPase activity poly(A) RNA binding mRNA 5'-UTR binding cadherin binding involved in cell-cell adhesion nucleotide binding nucleic acid binding helicase activity hydrolase activity
Process(es)
transcription, DNA-templated chromosome segregation extrinsic apoptotic signaling pathway via death domain receptors response to virus RNA secondary structure unwinding positive regulation of gene expression Wnt signaling pathway negative regulation of translation positive regulation of cell growth negative regulation of cell growth negative regulation of protein complex assembly DNA duplex unwinding positive regulation of interferon-beta production stress granule assembly intracellular signal transduction mature ribosome assembly positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of viral genome replication innate immune response positive regulation of translation positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation cellular response to arsenic-containing substance cellular response to osmotic stress positive regulation of chemokine (C-C motif) ligand 5 production intrinsic apoptotic signaling pathway cell-cell adhesion positive regulation of G1/S transition of mitotic cell cycle protein localization to cytoplasmic stress granule negative regulation of intrinsic apoptotic signaling pathway immune system process regulation of transcription, DNA-templated translational initiation regulation of translation apoptotic process regulation of gene expression ribosome biogenesis
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