Invitrogen

DDR2 Polyclonal Antibody

Name: DDR2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79144

View all (16) DDR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

DDR2 Antibody (PA5-79144) in WB

Western blot analysis of DDR2 in, Lane 1: human U87 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was i... View More

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Immunogen Image DDR2 Polyclonal Antibody

Product Details

PA5-79144

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DDR2 (834-855aa RDTKNRPSFQEIHLLLLQQGDE).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2746260

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human U87 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell.

Target Information

DDR2 is a member of a subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. DDR1 and DDR2 have been shown to be potently inhibited by Gleevec. DDR2 required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD167 antigen-like family member B; CD167b; CD167b antigen; CD_antigen: CD167b; cell migration-inducing protein 20; Discoidin domain receptor 2; discoidin domain receptor family, member 2; Discoidin domain-containing receptor 2; Discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; RP11-572K18.1; tyrosine-protein kinase TYRO 10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase

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Gene Aliases: AW495251; DDR2; MIG20a; NTRKR3; TKT; TYRO10

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UniProt ID: (Human) Q16832, (Mouse) Q62371

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Entrez Gene ID: (Human) 4921, (Mouse) 18214, (Rat) 685781

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Function(s)

transmembrane receptor protein tyrosine kinase activity protein binding collagen binding ATP binding protein tyrosine kinase collagen receptor activity nucleotide binding protein kinase activity protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

ossification endochondral bone growth cell adhesion signal transduction regulation of extracellular matrix disassembly positive regulation of fibroblast migration peptidyl-tyrosine phosphorylation extracellular matrix organization collagen fibril organization regulation of bone mineralization biomineral tissue development chondrocyte proliferation collagen-activated tyrosine kinase receptor signaling pathway positive regulation of osteoblast differentiation positive regulation of protein kinase activity protein autophosphorylation positive regulation of fibroblast proliferation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of extracellular matrix disassembly protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDR2 Polyclonal Antibody

DDR2 Antibody (PA5-79144) in WB

Product Details

PA5-79144

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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