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  • Primary Antibodies ›
  • DDIT4 Antibodies

Invitrogen

DDIT4 Polyclonal Antibody

View all (16) DDIT4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite DDIT4 Polyclonal Antibody

  • Antibody Testing Data (8)
DDIT4 Antibody in Immunocytochemistry (ICC/IF)
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DDIT4 Antibody in Immunocytochemistry (ICC/IF)
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DDIT4 Antibody (PA5-109213) in ICC/IF

Immunofluorescent analysis of DDIT4 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with DDIT4 polyclonal antibody (Product # PA5-109213), followed by DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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DDIT4 Antibody in Immunocytochemistry (ICC/IF)
DDIT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DDIT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DDIT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DDIT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DDIT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
DDIT4 Antibody in Western Blot (WB)
DDIT4 Antibody in Flow Cytometry (Flow)
DDIT4 Polyclonal Antibody

Product Details

PA5-109213

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within Human DDIT4 aa 183-232
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854624

Product Specific Information

Positive Control: Hela and K562 cell lysates, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, A549.

Target Information

DDIT4 gene ontology annotations related to this gene include cellular response to dexamethasone stimulus; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of glycolytic process; negative regulation of TOR signaling; protein-containing complex disassembly; reactive oxygen species metabolic process; response to hypoxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; RP11-442H21.1

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Gene Aliases: DDIT4; Dig2; REDD-1; REDD1; RTP801

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UniProt ID: (Human) Q9NX09

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Entrez Gene ID: (Human) 54541

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Function(s)
14-3-3 protein binding
Process(es)
response to hypoxia neuron migration brain development cell proliferation negative regulation of peptidyl-threonine phosphorylation neuron differentiation negative regulation of TOR signaling negative regulation of peptidyl-serine phosphorylation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator protein complex disassembly negative regulation of glycolytic process neurotrophin TRK receptor signaling pathway defense response to virus reactive oxygen species metabolic process positive regulation of neuron death negative regulation of intracellular signal transduction
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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