Invitrogen

Cytokeratin 17 Monoclonal Antibody (E3)

Name: Cytokeratin 17 Monoclonal Antibody (E3)
Brand: Invitrogen
SKU: MA1-06325

Advanced Verification

7 References

View all (53) Cytokeratin 17 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (2)

Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Cytokeratin 17 Antibody (MA1-06325) in ICC/IF

Immunofluorescence analysis of Cytokeratin 17 was performed using MCF 10A and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 17 Monoclonal Antibody (Product # MA1-06325) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degr... View More

Cytokeratin 17 Antibody in Western Blot (WB)

Product Image Gallery

Immunogen image

Immunogen Image Cytokeratin 17 Monoclonal Antibody (E3)

Product Details

MA1-06325

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:25-1:200

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Cytoskeletal preparation from rat colon.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_559766

Product Specific Information

MA1-06325 detects cytokeratin 17 in human and rat samples.

MA1-06325 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry.

The MA1-06325 immunogen is a cytoskeletal preparation from rat colon.

Target Information

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately40 kDa and approximately68 kDa and isoelectric pH between 4. 9 - 7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impaired wound repair in adult endoglin heterozygous mice associated with lower NO bioavailability.

The Journal of investigative dermatology

Pérez-Gómez E,Jerkic M,Prieto M,Del Castillo G,Martín-Villar E,Letarte M,Bernabeu C,Pérez-Barriocanal F,Quintanilla M,López-Novoa JM

MA1-06325 was used in immunohistochemistry to study the role of reduced NO levels in the defective skin wound healing observed in adult endoglin (+/-) heterozygous mice

Wed Jan 01 00:00:00 UTC 2014

CD200-expressing human basal cell carcinoma cells initiate tumor growth.

Proceedings of the National Academy of Sciences of the United States of America

Colmont CS,Benketah A,Reed SH,Hawk NV,Telford WG,Ohyama M,Udey MC,Yee CL,Vogel JC,Patel GK

MA1-06325 was used in immunohistochemistry to study the abiliy of the CD200-positive human basal cell carcinoma cells to initiate tumor growth

Tue Jan 22 00:00:00 UTC 2013

Triple-negative breast cancer: immunohistochemical correlation with basaloid markers and prognostic value of survivin.

Medical oncology (Northwood, London, England)

Dogu GG,Ozkan M,Ozturk F,Dikilitas M,Er O,Ozturk A

MA1-06325 was used in immunohistochemistry to study the prognostic value of survivin in patients with basaloid triplet-negative breast cancer

Mon Mar 01 00:00:00 UTC 2010

Omics-based profiling of carcinoma of the breast and matched regional lymph node metastasis.

Proteomics

Li J,Gromov P,Gromova I,Moreira JM,Timmermans-Wielenga V,Rank F,Wang K,Li S,Li H,Wiuf C,Yang H,Zhang X,Bolund L,Celis JE

MA1-06325 was used in immunohistochemistry to investigate the relationship between axillary lymph node metastases and primary breast tumors

Mon Dec 01 00:00:00 UTC 2008

Keratin profiling in the developing human prostate. A different approach to understanding epithelial lineage.

Anticancer research

Trompetter M,Smedts F,van der Wijk J,Schoots C,de Jong HJ,Hopman A,de la Rosette J

MA1-06325 was used in immunohistochemistry to investigate the distribution of keratin in prostate during development

Thu Apr 10 00:00:00 UTC 2008

Basoluminal carcinoma: a new biologically and prognostically distinct entity between basal and luminal breast cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Laakso M,Tanner M,Nilsson J,Wiklund T,Erikstein B,Kellokumpu-Lehtinen P,Malmström P,Wilking N,Bergh J,Isola J

MA1-06325 was used in immunohistochemistry to study the characteristics of basoluminal carcinoma

Sat Jul 15 00:00:00 UTC 2006

Cytokeratin expression in lichen amyloidosus and macular amyloidosis.

Journal of the European Academy of Dermatology and Venereology : JEADV

Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N

MA1-06325 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value

Sat May 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 39.1; CK-17; CK17; Cytokeratin-17; hard keratin; K17; keratin 17, type I; Keratin, type I cytoskeletal 17; Keratin-17; krt17 {ECO:0000250|UniProtKB:Q04695}; Type I keratin Ka17

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Gene Aliases: 39.1; CK-17; K17; Ka17; KRT17; PC; PC2; PCHC1

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UniProt ID: (Human) Q04695, (Rat) Q6IFU8

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Entrez Gene ID: (Human) 3872, (Rat) 287702

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Function(s)

structural constituent of cytoskeleton protein binding MHC class II receptor activity MHC class II protein binding

Process(es)

signal transduction epidermis development positive regulation of cell growth hair follicle morphogenesis keratinization intermediate filament organization positive regulation of translation positive regulation of hair follicle development morphogenesis of an epithelium intermediate filament-based process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin 17 Monoclonal Antibody (E3)

Cytokeratin 17 Antibody (MA1-06325) in ICC/IF

Product Details

MA1-06325

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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