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  • Cytokeratin 17 Antibodies

Huabio

Cytokeratin 17 Monoclonal Antibody (83-13)

View all (53) Cytokeratin 17 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Cytokeratin 17 Monoclonal Antibody (83-13)

  • Antibody Testing Data (6)
Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)
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Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)
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Cytokeratin 17 Antibody (M0407-13) in ICC/IF

ICC staining Cytokeratin 17 in SK-Br-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the se... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)
Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)
Cytokeratin 17 Antibody in Immunocytochemistry (ICC/IF)
Cytokeratin 17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytokeratin 17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytokeratin 17 Antibody in Western Blot (WB)
Cytokeratin 17 Monoclonal Antibody (83-13)

Product Details

M0407-13

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

83-13

Immunogen

Synthetic peptide within Human Cytokeratin 17 aa 383-432/432.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately40 kDa and approximately68 kDa and isoelectric pH between 4. 9 - 7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 39.1; CK-17; CK17; Cytokeratin-17; hard keratin; K17; keratin 17, type I; keratin complex 1, acidic, gene 17; Keratin, type I cytoskeletal 17; Keratin-17; krt17 {ECO:0000250|UniProtKB:Q04695}; Type I keratin Ka17

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Gene Aliases: 39.1; CK-17; K17; Ka17; Krt1-17; KRT17; PC; PC2; PCHC1

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UniProt ID: (Human) Q04695, (Mouse) Q9QWL7, (Rat) Q6IFU8

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Entrez Gene ID: (Human) 3872, (Mouse) 16667, (Rat) 287702

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Function(s)
structural constituent of cytoskeleton protein binding MHC class II receptor activity MHC class II protein binding structural molecule activity
Process(es)
morphogenesis of an epithelium signal transduction positive regulation of cell growth hair follicle morphogenesis keratinization intermediate filament organization positive regulation of translation positive regulation of hair follicle development epidermis development intermediate filament-based process
It has to be done as per old AB suggested Products section.
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