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  • Cytochrome C Antibodies

NeoBiotechnologies

Cytochrome C Recombinant Mouse Monoclonal Antibody (rCYCS, 1010)

View all (67) Cytochrome C antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Cytochrome C Recombinant Mouse Monoclonal Antibody (rCYCS, 1010)

  • Antibody Testing Data (4)
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Cytochrome C Antibody (54205-MSM7-P1ABX) in IHC (P)

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human liver using Cytochrome C Recombinant Monoclonal Antibody (Product # 54205-MSM7-P1ABX). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Western Blot (WB)
Cytochrome C Antibody in SDS-PAGE (SDS-PAGE)

Product Details

54205-MSM7-P1ABX

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Amphibian, Avian, Dog, Fruit fly, Horse, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

rCYCS, 1010

Immunogen

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CG17903-PA; CG17903-PB; CRF; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; cytochrome c proximal; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1

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Gene Aliases: CG17903; CYC; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; pDMc01; THC4

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UniProt ID: (Dog) P00011, (Human) P99999, (Horse) P00004, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029

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Entrez Gene ID: (Dog) 475258, (Human) 54205, (Horse) 100053958, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996

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Function(s)
heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding protein serine/threonine phosphatase activity protein binding electron carrier activity iron ion binding lipid binding
Process(es)
mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen apoptotic DNA fragmentation positive regulation of cysteine-type endopeptidase activity involved in apoptotic process response to reactive oxygen species protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport oxidative phosphorylation response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration
It has to be done as per old AB suggested Products section.
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