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  • Cytochrome C Antibodies

Invitrogen

Cytochrome C Recombinant Rabbit Monoclonal Antibody (SC59-01)

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View all (67) Cytochrome C antibodies

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Datasheet
Protocols
Questions & Answers

Cite Cytochrome C Recombinant Rabbit Monoclonal Antibody (SC59-01)

  • Antibody Testing Data (11)
  • Advanced Verification (1)
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Cytochrome C Antibody (MA5-41146) in IHC (P)

Immunohistochemical analysis of Cytochrome C was performed using formalin-fixed paraffin-embedded mouse kidney tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minute... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Cytochrome C Antibody in Western Blot (WB)
Cytochrome C Antibody in Western Blot (WB)
Cytochrome C Antibody in Western Blot (WB)
Cytochrome C Antibody
Cytochrome C Recombinant Rabbit Monoclonal Antibody (SC59-01)

Product Details

MA5-41146

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC59-01

Immunogen

Synthetic peptide within human Cytochrome C aa 10-50
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898900

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CRF; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1

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Gene Aliases: CYC; CYCS; CYCSA; HCS; THC4

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UniProt ID: (Human) A4D166, (Mouse) P62897, (Rat) P62898

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Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309

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Function(s)
protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding
Process(es)
response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration
It has to be done as per old AB suggested Products section.
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