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Cyclin B1 Monoclonal Antibody (C2-F2)

View all (54) Cyclin B1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
Cyclin B1 Monoclonal Antibody (C2-F2)

Product Details

M1508-1

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunocytochemistry (ICC/IF)

1:200-1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C2-F2

Immunogen

Synthetic peptide (KLH-coupled) within human Cyclin B1 aa 391-433.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023

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Gene Aliases: CCNB; CCNB1

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UniProt ID: (Human) P14635

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Entrez Gene ID: (Human) 891

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Function(s)
cyclin-dependent protein serine/threonine kinase activity patched binding protein binding protein kinase binding histone kinase activity
Process(es)
G2/M transition of mitotic cell cycle oocyte maturation in utero embryonic development negative regulation of protein phosphorylation protein complex assembly DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic spindle organization mitotic nuclear envelope disassembly mitotic metaphase plate congression spermatogenesis response to mechanical stimulus negative regulation of gene expression anaphase-promoting complex-dependent catabolic process positive regulation of mRNA 3'-end processing positive regulation of histone phosphorylation tissue regeneration response to drug protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of mitotic cell cycle response to DDT positive regulation of fibroblast proliferation digestive tract development cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of cell cycle positive regulation of attachment of spindle microtubules to kinetochore ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation regulation of chromosome condensation cellular response to iron(III) ion cellular response to fatty acid cellular response to organic cyclic compound cellular response to hypoxia regulation of mitotic cell cycle spindle assembly checkpoint histone H3-S10 phosphorylation involved in chromosome condensation
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