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  • Creatine Kinase BB Antibodies

Invitrogen

Creatine Kinase BB Polyclonal Antibody

View all (53) Creatine Kinase BB antibodies

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Datasheet
Protocols
Questions & Answers

Cite Creatine Kinase BB Polyclonal Antibody

  • Antibody Testing Data (4)
Creatine Kinase BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Creatine Kinase BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Creatine Kinase BB Antibody (PA5-79049) in IHC (P)

Immunohistochemistry analysis of Creatine Kinase BB on paraffin-embedded human colon cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Creatine Kinase BB polyclonal antibody (Product# PA5-79049) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit I... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Creatine Kinase BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Creatine Kinase BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Creatine Kinase BB Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Creatine Kinase BB Antibody in Western Blot (WB)
Creatine Kinase BB Polyclonal Antibody

Product Details

PA5-79049

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human CKB recombinant protein (Position: H7-K381).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746165

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human placenta tissue, human COLO-320 whole cell, human SW620 whole cell, human MDA-MB-231 whole cell, rat brain tissue, mouse brain tissue. IHC: human colon cancer tissue, mouse kidney tissue, rat kidney tissue.

Target Information

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-CK; Brain creatine kinase; CPK-B; Creatine kinase B chain; Creatine kinase B-type; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; Creatine phosphokinase B-type; Creatine phosphokinase M-type; epididymis luminal protein 211; epididymis secretory protein Li 29

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Gene Aliases: B-CK; BCK; Ck-3; Ck3; CKB; CKBB; Ckbr; HEL-211; HEL-S-29

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UniProt ID: (Human) P12277, (Rat) P07335, (Mouse) Q04447

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Entrez Gene ID: (Human) 1152, (Rat) 24264, (Mouse) 12709

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Function(s)
creatine kinase activity protein binding ATP binding ubiquitin protein ligase binding nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
creatine metabolic process phosphorylation substantia nigra development cellular chloride ion homeostasis phosphocreatine metabolic process brain development
It has to be done as per old AB suggested Products section.
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