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  • Complement C3 Antibodies

Invitrogen

Complement C3 Polyclonal Antibody

Advanced Verification
9 Published Figures
20 References
View all (29) Complement C3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Complement C3 Polyclonal Antibody

  • Antibody Testing Data (8)
  • Published Figures (9)
  • Advanced Verification (2)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
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Complement C3 Antibody in Immunocytochemistry (ICC/IF)
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Complement C3 Antibody (PA5-21349) in ICC/IF

Immunofluorescence analysis of Complement C3 was performed using 70% confluent log phase Hep G2 cells treated with 10 ng/mL of LPS for 18 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Complement C3 Polyclonal Antibody (Product # PA5-21349) at 5 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Immunoprecipitation (IP)
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunocytochemistry (ICC/IF)
Complement C3 Antibody in Immunohistochemistry (IHC)
Complement C3 Antibody in Western Blot (WB)
Complement C3 Antibody in Flow Cytometry (Flow)
Complement C3 Antibody
Complement C3 Antibody
Complement C3 Polyclonal Antibody

Product Details

PA5-21349

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 6 publications 6 publications

Immunohistochemistry (IHC)

-
View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
View 4 publications 4 publications

Flow Cytometry (Flow)

-
View 2 publications 2 publications

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153785

Product Specific Information

Recommended positive controls: Huh7, HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3

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Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

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UniProt ID: (Human) P01024, (Mouse) P01027

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Entrez Gene ID: (Human) 718, (Mouse) 12266

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Function(s)
serine-type endopeptidase activity endopeptidase inhibitor activity receptor binding protein binding lipid binding C5L2 anaphylatoxin chemotactic receptor binding cofactor binding
Process(es)
positive regulation of type IIa hypersensitivity positive regulation of protein phosphorylation positive regulation of activation of membrane attack complex tolerance induction proteolysis fatty acid metabolic process inflammatory response immune response complement activation complement activation, alternative pathway complement activation, classical pathway signal transduction G-protein coupled receptor signaling pathway blood coagulation positive regulation of vascular endothelial growth factor production positive regulation of glucose transport regulation of triglyceride biosynthetic process positive regulation of lipid storage negative regulation of endopeptidase activity regulation of complement activation response to magnesium ion response to estradiol response to progesterone response to estrogen positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of developmental growth regulation of immune response response to glucocorticoid positive regulation of ERK1 and ERK2 cascade positive regulation of apoptotic cell clearance
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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