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  • Chicken anti-Goat IgG Secondary Antibodies

Invitrogen

Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594

8 Published Figures
View all (15) Chicken anti-Goat IgG antibodies

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Datasheet
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Cite Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594

  • Antibody Testing Data (1)
  • Published Figures (8)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 9

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Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21468) in ICC/IF

Immunofluorescence analysis of Chicken anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594 (Product # A-21468) was performed using LNCaP cells stained with CD10 Polyclonal Antibody (Product # PA5-47075). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 2% BSA for 1 hour and labeled with 1:150 dilution of prim... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunocytochemistry (ICC/IF)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (IHC)
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Details

A-21468

Applications
Tested Dilution

Immunohistochemistry (IHC)

1-10 µg/mL

Immunocytochemistry (ICC/IF)

0.5-10 µg/mL
Product Specifications

Species Reactivity

Goat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594
  • Alexa Fluor 488 
  • Alexa Fluor 647 
  • AP
  • Biotin 
  • FITC
  • HRP
  • TRITC
  • Request custom conjugation

Excitation/Emission Max

590/618 nm View spectra spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2535871

Target

IgG

Cross Adsorption

Against human, mouse and rabbit IgG prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

This Chicken anti-goat antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from goat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic ″Fc″ domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.



Product will be shipped at Room Temperature.

Target Information

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody A-21468 on CiteAb

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