Invitrogen

ChAT Recombinant Rabbit Monoclonal Antibody (13H9L16)

Name: ChAT Recombinant Rabbit Monoclonal Antibody (13H9L16)
Brand: Invitrogen
SKU: 703789

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Datasheet

Protocols

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Antibody Testing Data (1)
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ChAT Antibody (703789) in WB

Western blot was performed using Anti-ChAT Recombinant Rabbit Monoclonal Antibody (Product # 703789) and a 70 kDa band corresponding to ChAT was observed in the relevant tissue models tested. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Kidney (Lane 5), Rat Kidney (Lane 6), Mouse Ovary (Lane 7), Mouse Lung (Lane 8) and Mouse Adipose (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then... View More

Product Image Gallery

ChAT Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Expression of ChAT was observed specifically in Brain and cerebellum in comparison to other tissues tested using Anti-ChAT Recombinant Rabbit Monoclonal Antibody (Product # 703789) in western blot. {RE}

Immunogen image

Immunogen Image ChAT Recombinant Rabbit Monoclonal Antibody (13H9L16)

Product Details

703789

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

13H9L16

Immunogen

Protein corresponding to human ChAT [aa275-aa374]

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2848239

Product Specific Information

This antibody is predicted to react with Bovine.

Target Information

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; Choline O-acetyltransferase

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Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6

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UniProt ID: (Human) P28329

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Entrez Gene ID: (Human) 1103, (Rat) 290567, (Mouse) 12647

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Function(s)

choline O-acetyltransferase activity choline binding transferase activity transferase activity, transferring acyl groups

Process(es)

antral ovarian follicle growth response to hypoxia phosphatidylcholine biosynthetic process neurotransmitter secretion response to nutrient memory acetylcholine biosynthetic process response to drug response to ethanol chemical synaptic transmission neuromuscular synaptic transmission muscle organ development establishment of synaptic specificity at neuromuscular junction rhythmic behavior adult walking behavior metabolic process dendrite development neuron differentiation neurotransmitter biosynthetic process rhythmic excitation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ChAT Recombinant Rabbit Monoclonal Antibody (13H9L16)

ChAT Antibody (703789) in WB

Product Details

703789

Western Blot (WB)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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