Invitrogen

Cdc5L Recombinant Rabbit Monoclonal Antibody (JJ091-05)

Name: Cdc5L Recombinant Rabbit Monoclonal Antibody (JJ091-05)
Brand: Invitrogen
SKU: MA5-41197

View all (20) CDC5L antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)

Cdc5L Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Cdc5L Antibody (MA5-41197) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of CDC5L in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cdc5L Recombinant Monoclonal Antibody (Product # MA5-41197) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Cdc5L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image Cdc5L Recombinant Rabbit Monoclonal Antibody (JJ091-05)

Product Details

MA5-41197

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ091-05

Immunogen

Synthetic peptide within human CDC5L aa 320-370

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2898951

Target Information

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDC5 cell division cycle 5-like; Cdc5-like protein; Cdc5-related protein; Cell division cycle 5-like protein; cell division cycle 5-related protein; dJ319D22.1 (CDC5-like protein); hCDC5; Pombe Cdc5-related protein

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Gene Aliases: 1200002I02Rik; AA408004; CDC5; CDC5-LIKE; CDC5L; CEF1; dJ319D22.1; KIAA0432; PCDC5RP

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UniProt ID: (Human) Q99459, (Rat) O08837, (Mouse) Q6A068

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Entrez Gene ID: (Human) 988, (Rat) 85434, (Mouse) 71702

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor recruiting transcription corepressor binding DNA binding protein binding protein phosphatase 1 binding protein kinase binding leucine zipper domain binding sequence-specific DNA binding transcription regulatory region DNA binding poly(A) RNA binding WD40-repeat domain binding chromatin binding protein domain specific binding RNA binding

Process(es)

G2/M transition of mitotic cell cycle DNA repair transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing RNA splicing signal transduction involved in DNA damage checkpoint mitotic cell cycle mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter cell differentiation cellular response to fibroblast growth factor stimulus cellular response to interleukin-2 cellular response to wortmannin cellular response to nerve growth factor stimulus cellular response to prolactin cellular response to DNA damage stimulus cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc5L Recombinant Rabbit Monoclonal Antibody (JJ091-05)

Cdc5L Antibody (MA5-41197) in ICC/IF

Product Details

MA5-41197

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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