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  • Primary Antibodies ›
  • CDC5L Antibodies

Invitrogen

Cdc5L Polyclonal Antibody

View all (20) CDC5L antibodies

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Datasheet
Protocols
Questions & Answers

Cite Cdc5L Polyclonal Antibody

  • Antibody Testing Data (1)
Cdc5L Antibody in Western Blot (WB)
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Cdc5L Antibody in Western Blot (WB)
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Cdc5L Antibody (PA5-95132) in WB

Western blot analysis of CDC5L in, Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat C6 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane wa... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Cdc5L Antibody in Western Blot (WB)
Cdc5L Polyclonal Antibody

Product Details

PA5-95132

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CDC5L (782-802aa KELQHRYADLLLEKETLKSKF).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806937

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HEK293 whole cell, human THP-1 whole cell, rat C6 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDC5 cell division cycle 5-like; Cdc5-like protein; Cdc5-related protein; Cell division cycle 5-like protein; cell division cycle 5-related protein; dJ319D22.1 (CDC5-like protein); hCDC5; Pombe Cdc5-related protein

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Gene Aliases: CDC5; CDC5-LIKE; CDC5L; CEF1; dJ319D22.1; KIAA0432; PCDC5RP

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UniProt ID: (Human) Q99459, (Rat) O08837

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Entrez Gene ID: (Human) 988, (Rat) 85434

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor recruiting transcription corepressor binding DNA binding protein binding protein phosphatase 1 binding protein kinase binding leucine zipper domain binding sequence-specific DNA binding transcription regulatory region DNA binding poly(A) RNA binding WD40-repeat domain binding chromatin binding protein domain specific binding
Process(es)
G2/M transition of mitotic cell cycle DNA repair transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing RNA splicing signal transduction involved in DNA damage checkpoint mitotic cell cycle mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter cell differentiation cellular response to fibroblast growth factor stimulus cellular response to interleukin-2 cellular response to wortmannin cellular response to nerve growth factor stimulus cellular response to prolactin
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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