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  • Primary Antibodies ›
  • Calponin 2/3 Antibodies

Bioss

Calponin 2 + 3 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Calponin 2 + 3 Polyclonal Antibody

  • Antibody Testing Data (2)
Calponin 2 + 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Calponin 2 + 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Calponin 2 + 3 Antibody (BS-4902R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Calponin 2 + 3 Polyclonal Antibody, Unconjugated (bs-4902R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Calponin 2 + 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Calponin 2 + 3 Antibody in Flow Cytometry (Flow)

Product Details

BS-4902R

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

1:500-1:1,000
-

Immunoelectrophoresis (IE)

1:20-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Calponin 2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acidic calponin h3; calponin 2; calponin 3, acidic; Calponin H2, smooth muscle; Calponin, acidic isoform; Calponin, non-muscle isoform; Calponin-2; Calponin-3; dJ639P13.2.2 (acidic calponin 3); h2-calponin; Neutral calponin

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Gene Aliases: 1600014M03Rik; AA408047; AI324678; C85854; Calpo2; Calpo3; CNN2; CNN3

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UniProt ID: (Human) Q99439, (Human) Q15417, (Mouse) Q08093, (Mouse) Q9DAW9, (Rat) P37397

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Entrez Gene ID: (Human) 1265, (Human) 1266, (Mouse) 12798, (Rat) 690976, (Mouse) 71994, (Rat) 54321

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Function(s)
actin binding calmodulin binding cadherin binding involved in cell-cell adhesion microtubule binding protein binding protein binding, bridging
Process(es)
cytoskeleton organization positive regulation of gene expression hemopoiesis negative regulation of cell migration actomyosin structure organization regulation of actin filament-based process wound healing regulation of cell proliferation negative regulation of phagocytosis cellular response to mechanical stimulus epithelial cell differentiation negative regulation of ATPase activity cell-cell adhesion
It has to be done as per old AB suggested Products section.
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