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  • Caldesmon Antibodies

Invitrogen

Caldesmon HMW Monoclonal Antibody (h-CALD)

Advanced Verification
2 References
View all (50) Caldesmon antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Caldesmon HMW Monoclonal Antibody (h-CALD)

  • Antibody Testing Data (6)
  • Advanced Verification (2)
Caldesmon HMW Antibody in Immunocytochemistry (ICC/IF)
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Caldesmon HMW Antibody in Immunocytochemistry (ICC/IF)
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Caldesmon HMW Antibody (MA5-11626) in ICC/IF

Immunofluorescence analysis of Caldesmon was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caldesmon HMW Monoclonal Antibody (h-CALD) (Product # MA5-11626) at 1:100 dilution in 0.1% BSA, incubated at 4 de... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Caldesmon HMW Antibody in Immunocytochemistry (ICC/IF)
Caldesmon HMW Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Caldesmon HMW Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Caldesmon HMW Antibody in Western Blot (WB)
Caldesmon HMW Antibody in Western Blot (WB)
Caldesmon HMW Antibody in Western Blot (WB)
Caldesmon HMW Antibody
Caldesmon HMW Antibody
Caldesmon HMW Monoclonal Antibody (h-CALD)

Product Details

MA5-11626

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
-

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400
-

Immunocytochemistry (ICC/IF)

1:10-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

h-CALD

Immunogen

Crude human uterus extract
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980916

Product Specific Information

MA5-11626 targets Caldesmon HMW in IHC (P), IF and WB applications and shows reactivity with human and mouse samples.

The MA5-11626 immunogen is crude human uterus extract.

Target Information

Caldesmon is a developmentally regulated protein involved in smooth muscle and non muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their electrophorectic mobility and cellular distribution. The h caldesmon variant (120 - 150 kDa) is predominantly expressed in smooth muscle whereas l caldesmon (70 - 80 kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Caldesmon; CDM; h-caldesmon; L-caldesmon; MGC21352; Non-muscle caldesmon; OTTHUMP00000207950; OTTHUMP00000207953; OTTHUMP00000207954; OTTHUMP00000207955; OTTHUMP00000214473; testis secretory sperm-binding protein Li 227n

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Gene Aliases: 4833423D12Rik; AI195384; AV071549; AW536160; C920027I18Rik; CAD; CALD1; CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22

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UniProt ID: (Human) Q05682, (Rat) Q62736

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Entrez Gene ID: (Human) 800, (Rat) 25687, (Mouse) 109624

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Function(s)
actin binding protein binding calmodulin binding tropomyosin binding myosin binding cadherin binding involved in cell-cell adhesion
Process(es)
movement of cell or subcellular component muscle contraction cell-cell adhesion female pregnancy positive regulation of protein binding actin filament bundle assembly response to transforming growth factor beta regulation of smooth muscle contraction
It has to be done as per old AB suggested Products section.
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