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  • Primary Antibodies ›
  • CXCL1 (GRO alpha) Antibodies
Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Invitrogen

CXCL1 Recombinant Superclonal™ Antibody (14HCLC)

Advanced Verification
1 Published Figure
1 Reference
View all (31) CXCL1 (GRO alpha) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CXCL1 Recombinant Superclonal™ Antibody (14HCLC)

  • Antibody Testing Data (1)
  • Published Figures (1)
  • Advanced Verification (1)
CXCL1 Antibody in Immunocytochemistry (ICC/IF)
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CXCL1 Antibody in Immunocytochemistry (ICC/IF)
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CXCL1 Antibody (712317) in ICC/IF

For immunofluorescence analysis, A375 cells were fixed and permeabilized for detection of endogenous CXCL1 using Anti-CXCL1 Recombinant Rabbit Superclonal™ Antibody (Product # 712317, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows represent... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CXCL1 Antibody in Immunocytochemistry (ICC/IF)
CXCL1 Antibody in Immunocytochemistry (ICC/IF)
CXCL1 Antibody
CXCL1 Recombinant Superclonal™ Antibody (14HCLC)

Product Details

712317

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:100
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

14HCLC

Immunogen

Peptide corresponding to human CXCL1 [aa99-aa107]
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2784615

Product Specific Information

This Antibody is predicted to react with Monkey, Pig, Sheep, Bovine.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3

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Gene Aliases: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1

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UniProt ID: (Human) P09341

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Entrez Gene ID: (Human) 2919

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Function(s)
receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding
Process(es)
chemotaxis inflammatory response immune response signal transduction G-protein coupled receptor signaling pathway nervous system development cell proliferation negative regulation of cell proliferation actin cytoskeleton organization response to lipopolysaccharide intracellular signal transduction positive regulation of catalytic activity cell chemotaxis chemokine-mediated signaling pathway positive regulation of neutrophil chemotaxis
It has to be done as per old AB suggested Products section.
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