Invitrogen

CTCF Polyclonal Antibody

1 Published Figure
2 References
View all (28) CTCF antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
CTCF Polyclonal Antibody

Product Details

PA5-88115

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_0065561)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.18 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2804662

Product Specific Information

Immunogen sequence: MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV NAEKVVGNMK PPKPTKIKKK; Positive Samples: 293T, Jurkat; Cellular Location: Chromosome, Nucleus, centromere, nucleoplasm

Target Information

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF

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Gene Aliases: AW108038; CTCF; MRD21

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UniProt ID: (Human) P49711, (Rat) Q9R1D1, (Mouse) Q61164

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Entrez Gene ID: (Human) 10664, (Rat) 83726, (Mouse) 13018

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding zinc ion binding chromatin insulator sequence binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding chromatin binding metal ion binding nucleic acid binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter DNA methylation regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter chromosome segregation maintenance of DNA methylation positive regulation of gene expression negative regulation of gene expression chromatin modification nucleosome positioning regulation of histone methylation regulation of histone acetylation regulation of gene expression, epigenetic regulation of molecular function, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter covalent chromatin modification regulation of centromeric sister chromatid cohesion dosage compensation by inactivation of X chromosome
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