OriGene

CRY1 Monoclonal Antibody (OTI6C6), TrueMAB™

Name: CRY1 Monoclonal Antibody (OTI6C6), TrueMAB\u2122
Brand: OriGene
SKU: CF809967

View all (16) CRY1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

CRY1 Antibody (CF809967) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY1 (RC207152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY1. (1. Positive lysates LY418241 (100 µg) and LC418241 (20 µg) can be purchased separately from OriGene.

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Immunogen image

Immunogen Image CRY1 Monoclonal Antibody (OTI6C6), TrueMAB™

Product Details

CF809967

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI6C6

Immunogen

Human recombinant protein fragment corresponding to amino acids 502-584 of human CRY1 produced in E.coli.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cryptochrome 1 (photolyase-like); Cryptochrome-1

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Gene Aliases: AU020726; AU021000; CRY1; PHLL1

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UniProt ID: (Human) Q16526, (Rat) Q32Q86, (Mouse) P97784

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Entrez Gene ID: (Human) 1407, (Rat) 299691, (Mouse) 12952

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Function(s)

nucleotide binding transcription factor activity, transcription factor binding core promoter sequence-specific DNA binding core promoter binding DNA binding double-stranded DNA binding deoxyribodipyrimidine photo-lyase activity DNA (6-4) photolyase activity protein binding transcription factor binding blue light photoreceptor activity protein kinase binding phosphatase binding nuclear hormone receptor binding histone deacetylase binding ubiquitin binding DNA photolyase activity photoreceptor activity kinase binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter gluconeogenesis DNA repair transcription, DNA-templated DNA damage induced protein phosphorylation signal transduction circadian rhythm protein-chromophore linkage lipid storage negative regulation of protein ubiquitination response to insulin circadian regulation of gene expression response to glucagon glucose homeostasis regulation of circadian rhythm negative regulation of circadian rhythm entrainment of circadian clock by photoperiod negative regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated regulation of DNA damage checkpoint negative regulation of glucocorticoid receptor signaling pathway negative regulation of glucocorticoid secretion blue light signaling pathway regulation of transcription, DNA-templated rhythmic process response to stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CRY1 Monoclonal Antibody (OTI6C6), TrueMAB™

CRY1 Antibody (CF809967) in WB

Product Details

CF809967

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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