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  • Primary Antibodies ›
  • CPSF6 Antibodies

Bethyl Laboratories

CPSF68 Polyclonal Antibody

View all (14) CPSF6 antibodies

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Questions & Answers

Cite CPSF68 Polyclonal Antibody

  • Antibody Testing Data (1)
CPSF68 Antibody in Western Blot (WB)
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CPSF68 Antibody in Western Blot (WB)
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CPSF68 Antibody (A301-357A) in WB

Detection of human and mouse CPSF68 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CPSF68 antibody (Product # A301-357A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. CPSF68 was also immunoprecipitated by rabbit anti-CPSF68 antibodies A301-356A and A301-358A, which recognize other epitopes. Detection: Chemilumines... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CPSF68 Antibody in Western Blot (WB)
CPSF68 Polyclonal Antibody

Product Details

A301-357A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunoprecipitation (IP)

2-5 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 400 and 450 of human cleavage and polyadenylation specific factor 6, 68kDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan

Target Information

CPSF6 is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CFIm68; cleavage and polyadenylation specific factor 6, 68kDa; Cleavage and polyadenylation specificity factor 68 kDa subunit; Cleavage and polyadenylation specificity factor subunit 6; Cleavage factor Im complex 68 kDa subunit; CPSF 68 kDa subunit; pre-mRNA cleavage factor I, 68kD subunit; pre-mRNA cleavage factor Im (68kD); Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII-4/7

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Gene Aliases: 4733401N12Rik; AI256641; CFIM; CFIM68; CPSF6; HPBRII-4; HPBRII-7

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UniProt ID: (Human) Q16630, (Mouse) Q6NVF9

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Entrez Gene ID: (Human) 11052, (Mouse) 432508

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Function(s)
nucleotide binding RNA binding mRNA binding protein binding poly(A) RNA binding nucleic acid binding
Process(es)
mRNA polyadenylation mRNA processing protein tetramerization
It has to be done as per old AB suggested Products section.
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