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  • Primary Antibodies ›
  • COL4A2 Antibodies

Invitrogen

COL4A2 Polyclonal Antibody

1 Reference
View all (6) COL4A2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite COL4A2 Polyclonal Antibody

  • Antibody Testing Data (6)
COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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COL4A2 Antibody (PA5-104252) in IHC (P)

Immunohistochemistry analysis of COL4A2 in human pancreatic cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with COL4A2 polyclonal antibody (Product # PA5-104252) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COL4A2 Antibody in Western Blot (WB)
COL4A2 Antibody in Western Blot (WB)
COL4A2 Polyclonal Antibody

Product Details

PA5-104252

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human COL4A2(Accession P08572), corresponding to amino acid residues F155-G205.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2853568

Product Specific Information

Antibody detects endogenous levels of total Collagen IV alpha 2.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha-2 collagen type IV; canstatin; Collagen alpha-2(IV) chain; procollagen, type IV, alpha 2

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Gene Aliases: Col4a-2; COL4A2; ICH; POREN2

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UniProt ID: (Human) P08572, (Mouse) P08122

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Entrez Gene ID: (Human) 1284, (Rat) 306628, (Mouse) 12827

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Function(s)
extracellular matrix structural constituent
Process(es)
transcription, DNA-templated negative regulation of angiogenesis endodermal cell differentiation cellular response to transforming growth factor beta stimulus angiogenesis
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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