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  • Primary Antibodies ›
  • COBRA1 Antibodies

Invitrogen

COBRA1 Polyclonal Antibody

View all (13) COBRA1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite COBRA1 Polyclonal Antibody

  • Antibody Testing Data (5)
COBRA1 Antibody in Immunocytochemistry (ICC/IF)
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COBRA1 Antibody in Immunocytochemistry (ICC/IF)
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COBRA1 Antibody (PA5-118113) in ICC/IF

Immunofluorescence staining of COBRA1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with COBRA1 Polyclonal Antibody (Product # PA5-118113, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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COBRA1 Antibody in Immunocytochemistry (ICC/IF)
COBRA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COBRA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
COBRA1 Antibody in Western Blot (WB)
COBRA1 Antibody in Immunoprecipitation (IP)

Product Details

PA5-118113

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Immunoprecipitation (IP)

1-5 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human COBRA1/NELFB fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2902714

Target Information

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2), either NELFC or NELFD (TH1L), and NELFE (RDBP), a heterodimer of SPT4 (SUPT4H1), to cause transcriptional pausing of RNA polymerase II (Narita et al., 2003).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cofactor of BRCA1; DKFZp586B0519; Negative elongation factor B; negative elongation factor protein B; NELF-B; RP13-122B23.3

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Gene Aliases: COBRA1; KIAA1182; NELF-B; NELFB

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UniProt ID: (Human) Q8WX92

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Entrez Gene ID: (Human) 25920

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Function(s)
protein binding
Process(es)
transcription from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter negative regulation of transcription elongation from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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