Invitrogen

CNR2 Polyclonal Antibody

Name: CNR2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-744

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6 Published Figures

16 References

View all (14) CNR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 8

CNR2 Antibody (PA1-744) in WB

Western blot analysis of Cannabinoid Receptor II was performed by loading 25 µg of PC-3 (lane 1) and Jurkat (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Cannabinoid Receptor II polyclonal antibody (Product # PA1-744) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Pl... View More

CNR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CNR2 Antibody — Fig. 8 CB1 and CB2 expression, represented as median fluorescence intensity (left panel) in PBMC from 12 healthy controls and 12 MS patients under the specified stimulation conditions. Data are shown for lymphocyte/monocyte-gated cells. The right panel is a representative histogram of CB 1 and CB 2 expression in an MS patient's PBMCs in different stimulation conditions. Colour legend, Black: Isotype Control; Red: Unstimulated; Orange: +IL-1beta; Blue: +IL-6; Pink: +TNF-alpha. Results show a statistically significant increase in CB 2 expression between controls and MS patients after stimulation with IL-1beta, IL-6, and TNF-alpha. {TM}

Immunogen image

Immunogen Image CNR2 Polyclonal Antibody

Product Details

PA1-744

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:2500

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

1:50

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Horse, Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A fusion protein containing the first 33 amino acid residues from human CB2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2082910

Product Specific Information

PA1-744 detects cannabinoid receptor 2 (CB2) from human tissues.

PA1-744 has been successfully used in Western blot, FACS, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa band from human spinal cord homogenate, and a ~40 kda band from RBL2H3 cells, representing the different glycosylated forms of CB2.

PA1-744 antigen is a fusion protein containing the first 33 amino acid residues from human CB2.

Target Information

Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cannabinoid Receptor 2; cannabinoid receptor 2 (macrophage); CB-2; CB2R; CNR3; CX5; HCB2; HGNC:2160; OTTHUMP00000044841; RP11-4M23.1; testis-dominant CNR2 isoform CB2

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Gene Aliases: CB-2; CB2; CB2A; CB2B; CNR2; CX5

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UniProt ID: (Human) P34972

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Entrez Gene ID: (Human) 1269

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Function(s)

cannabinoid receptor activity

Process(es)

response to amphetamine inflammatory response immune response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger sensory perception of pain negative regulation of synaptic transmission, GABAergic response to lipopolysaccharide negative regulation of mast cell activation cannabinoid signaling pathway negative regulation of action potential negative regulation of inflammatory response negative regulation of nitric-oxide synthase activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CNR2 Polyclonal Antibody

CNR2 Antibody (PA1-744) in WB

Product Details

PA1-744

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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