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  • CK2 alpha-1 Antibodies

Bethyl Laboratories

CKII alpha Polyclonal Antibody

View all (30) CK2 alpha-1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite CKII alpha Polyclonal Antibody

  • Antibody Testing Data (3)
CKII alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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CKII alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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CKII alpha Antibody (A300-197A) in IHC (P)

Detection of human CKII Alpha by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha (Product # A300-197A) (Lot 2) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CKII alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
CKII alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
CKII alpha Antibody in Western Blot (WB)
CKII alpha Polyclonal Antibody

Product Details

A300-197A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residues 300 and 350 of human Casein Kinase 2, alpha 1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Chicken, Bovine and Rabbit

Target Information

CSNK2A1 is a serine/threonine protein kinase that phosphorylates acidic proteins. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by CK2 alpha-1 represents the alpha subunit. While CK2 alpha-1 is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for CK2 alpha-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 polypeptide pseudogene; casein kinase II alpha 1 subunit; Casein kinase II subunit alpha; casein kinase II, alpha 1 polypeptide; casein kinase II, alpha 1 related sequence 4; CK II alpha; CK II alpha 3; CK2 catalytic subunit alpha; protein kinase CK2

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Gene Aliases: CK2A1; CKII; Ckiia; CSNK2A1; Csnk2a1-rs4; CSNK2A3

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UniProt ID: (Human) P68400, (Mouse) Q60737

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Entrez Gene ID: (Human) 1457, (Mouse) 12995

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Function(s)
protein serine/threonine kinase activity protein binding ATP binding beta-catenin binding protein phosphatase regulator activity protein N-terminus binding Hsp90 protein binding kinase activity
Process(es)
protein phosphorylation positive regulation of cell proliferation positive regulation of Wnt signaling pathway positive regulation of cell growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of protein catabolic process protein autophosphorylation regulation of catalytic activity mitotic spindle checkpoint transcription, DNA-templated regulation of transcription, DNA-templated protein folding apoptotic process signal transduction Wnt signaling pathway rhythmic process chaperone-mediated protein folding regulation of signal transduction by p53 class mediator
It has to be done as per old AB suggested Products section.
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