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  • Primary Antibodies ›
  • CK2 alpha-1 Antibodies

Invitrogen

CK2 alpha-1 Polyclonal Antibody

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View all (30) CK2 alpha-1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite CK2 alpha-1 Polyclonal Antibody

  • Antibody Testing Data (2)
  • Advanced Verification (1)
CK2 alpha-1 Antibody in Western Blot (WB)
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CK2 alpha-1 Antibody in Western Blot (WB)
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CK2 alpha-1 Antibody (PA5-17534) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), U-87 MG (Lane 3), HCT 116 (Lane 4), MCF7 (Lane 5) and MKN45 (Lane 6). The blot was probed with Anti-CK2 alpha Polyclonal Antibody (Product # PA5-17534, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 44 kDa band corresponding to CK2 alpha was observed across the cell lines tested. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CK2 alpha-1 Antibody in Western Blot (WB)
CK2 alpha-1 Antibody in Western Blot (WB)
CK2 alpha-1 Antibody
CK2 alpha-1 Polyclonal Antibody

Product Details

PA5-17534

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acid residues near the carboxy-terminus of human CK2a1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11004650

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

CSNK2A1 is a serine/threonine protein kinase that phosphorylates acidic proteins. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by CK2 alpha-1 represents the alpha subunit. While CK2 alpha-1 is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for CK2 alpha-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 polypeptide pseudogene; casein kinase II alpha 1 subunit; Casein kinase II subunit alpha; casein kinase II, alpha 1 polypeptide; casein kinase II, alpha 1 related sequence 4; CK II alpha; CK II alpha 3; CK2 catalytic subunit alpha; protein kinase CK2

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Gene Aliases: CK2A1; CKII; Ckiia; CSNK2A1; Csnk2a1-rs4; CSNK2A3

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UniProt ID: (Human) P68400, (Mouse) Q60737, (Rat) P19139

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Entrez Gene ID: (Human) 1457, (Mouse) 12995, (Rat) 116549

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Function(s)
protein serine/threonine kinase activity protein binding ATP binding beta-catenin binding protein phosphatase regulator activity protein N-terminus binding Hsp90 protein binding kinase activity
Process(es)
protein phosphorylation positive regulation of cell proliferation positive regulation of Wnt signaling pathway positive regulation of cell growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of protein catabolic process protein autophosphorylation regulation of catalytic activity mitotic spindle checkpoint regulation of cell growth transcription, DNA-templated regulation of transcription, DNA-templated Wnt signaling pathway regulation of cell proliferation rhythmic process protein folding apoptotic process signal transduction chaperone-mediated protein folding regulation of signal transduction by p53 class mediator
It has to be done as per old AB suggested Products section.
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