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  • CK1 alpha Antibodies

Invitrogen

CK1 alpha Polyclonal Antibody

View all (17) CK1 alpha antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite CK1 alpha Polyclonal Antibody

  • Antibody Testing Data (3)
CK1 alpha Antibody in Immunocytochemistry (ICC/IF)
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CK1 alpha Antibody in Immunocytochemistry (ICC/IF)
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CK1 alpha Antibody (PA5-114945) in ICC/IF

Immunocytochemistry analysis of CK1 alpha in HeLa. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114945) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CK1 alpha Antibody in Immunocytochemistry (ICC/IF)
CK1 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
CK1 alpha Antibody in Western Blot (WB)
CK1 alpha Polyclonal Antibody

Product Details

PA5-114945

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CSNK1A1(Accession P48729), corresponding to amino acid residues K138-A188.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2899581

Product Specific Information

Antibody detects endogenous levels of total Casein Kinase 1 alpha.

Target Information

CNSK1A1 (CK1 alpha 1) is a member of the casein kinase I (CKI) gene family, which encodes serine/threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric, ranging from 25 to 55 kD, and are found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1, alpha 1; Casein kinase I isoform alpha; casein kinase I-alpha; CK I alpha; CK-I alpha; CK1; CKI alpha; CKI-alpha; clock regulator kinase; CSNK1A1/CK1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; OTTHUMP00000224000; OTTHUMP00000224001; OTTHUMP00000224003; YIL035C

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Gene Aliases: 2610208K14Rik; 4632404G05Rik; 5430427P18Rik; CK1; CK1a; CKIa; Csnk1a; CSNK1A1; HEL-S-77p; HLCDGP1; PRO2975

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UniProt ID: (Human) P48729, (Rat) P97633, (Mouse) Q8BK63

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Entrez Gene ID: (Human) 1452, (Rat) 113927, (Mouse) 93687

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity magnesium ion binding glycoprotein binding peptide binding phosphoprotein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
cell morphogenesis protein phosphorylation phagocytosis mitotic nuclear division regulation of cell shape Wnt signaling pathway peptidyl-serine phosphorylation cell division Golgi organization signal transduction cell surface receptor signaling pathway positive regulation of proteasomal ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process intermediate filament cytoskeleton organization negative regulation of canonical Wnt signaling pathway regulation of GTP binding beta-catenin destruction complex assembly beta-catenin destruction complex disassembly cell cycle phosphorylation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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