Invitrogen

CFTR Monoclonal Antibody (CF3)

Name: CFTR Monoclonal Antibody (CF3)
Brand: Invitrogen
SKU: MA1-935

3 Published Figures

7 References

View all (62) CFTR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (3)

CFTR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

CFTR Antibody (MA1-935) in ICC/IF

Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product # MA1-935) shows staining in WiDr colon carcinoma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product # MA1-935) at a dilution of 1:100-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ... View More

CFTR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Immunogen image

Immunogen Image CFTR Monoclonal Antibody (CF3)

Product Details

MA1-935

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

1/100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

CF3

Immunogen

Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2081230

Product Specific Information

MA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues.

MA1-935 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a single ~170 kDa protein representing CFTR in T84 whole cell extract. Immunofluorescence staining of CFTR in mouse epithelial cells with MA1-935 results in cell surface staining, consistent with localization at the plasma membrane. This antibody also detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA. MA1-935 can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines.

MA1-935 immunizing peptide corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis.

Target Information

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

MA1-935 was used in Western Blot, Flow Cytometry to suggest an important role for the CFTR-S1P axis in HF-mediated systemic and pulmonary inflammation.

Fri Jan 14 00:00:00 UTC 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

MA1-935 was used in Western Blot, Flow Cytometry to suggest an important role for the CFTR-S1P axis in HF-mediated systemic and pulmonary inflammation.

Fri Jan 14 00:00:00 UTC 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

MA1-935 was used in Western Blot, Flow Cytometry to suggest an important role for the CFTR-S1P axis in HF-mediated systemic and pulmonary inflammation.

Fri Jan 14 00:00:00 UTC 2022

Restoring myocardial infarction-induced long-term memory impairment by targeting the cystic fibrosis transmembrane regulator.

EBioMedicine

Vanherle L,Lidington D,Uhl FE,Steiner S,Vassallo S,Skoug C,Duarte JMN,Ramu S,Uller L,Desjardins JF,Connelly KA,Bolz SS,Meissner A

MA1-935 was used in Immunohistochemistry-immunofluorescence to indicate that CFTR therapeutics improve inflammation-induced alterations in hippocampal neuronal structure and attenuate memory dysfunction following MI.

Thu Dec 01 00:00:00 UTC 2022

Restoring myocardial infarction-induced long-term memory impairment by targeting the cystic fibrosis transmembrane regulator.

EBioMedicine

Vanherle L,Lidington D,Uhl FE,Steiner S,Vassallo S,Skoug C,Duarte JMN,Ramu S,Uller L,Desjardins JF,Connelly KA,Bolz SS,Meissner A

Thu Dec 01 00:00:00 UTC 2022

Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.

PloS one

Di Fulvio M,Bogdani M,Velasco M,McMillen TS,Ridaura C,Kelly L,Almutairi MM,Kursan S,Sajib AA,Hiriart M,Aguilar-Bryan L

MA1-935 was used in Immunohistochemistry to investigate the pathogenesis of cystic fibrosis-related diabetes using normal human islet cells.

Tue Jan 12 00:00:00 UTC 2021

Type IV(B) pili are required for invasion but not for adhesion of Salmonella enterica serovar Typhi into BHK epithelial cells in a cystic fibrosis transmembrane conductance regulator-independent manner.

Microbial pathogenesis

Bravo D,Blondel CJ,Hoare A,Leyton L,Valvano MA,Contreras I

MA1-935 was used in blocking or activating experiment to study the mechanism for Salmonella enterica serovar Typhi adhesion with BHK epithelial cells

Tue Nov 01 00:00:00 UTC 2011

Exaggerated apoptosis and NF-kappaB activation in pancreatic and tracheal cystic fibrosis cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Rottner M,Kunzelmann C,Mergey M,Freyssinet JM,Martínez MC

MA1-935 was used in immunohistochemistry to investigate the possible effect of CFTR mutations in pancreatic and tracheal cells on activation of apoptosis

Sat Sep 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: ATP Binding Cassette Superfamily C Member 7 (ABCC7); ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; ATP-binding cassette, subfamily c, member 7; cAMP-dependent chloride channel; CFTR; Channel conductance-controlling ATPase; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); cystic fibrosis transmembrane conductance regulator homolog; cystic fibrosis transmembrane conductance regulator homolog; ATP-binding cassette, subfamily c, member 7; tcag7.78; TNR CFTR

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Gene Aliases: ABC35; ABCC7; AW495489; CF; CFTR; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR

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UniProt ID: (Human) P13569, (Mouse) P26361

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Entrez Gene ID: (Human) 1080, (Mouse) 12638

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Function(s)

ATP-binding and phosphorylation-dependent chloride channel activity chloride channel activity channel-conductance-controlling ATPase activity protein binding ATP binding bicarbonate transmembrane transporter activity chloride transmembrane transporter activity chloride channel regulator activity chloride channel inhibitor activity enzyme binding PDZ domain binding anion transmembrane-transporting ATPase activity nucleotide binding hydrolase activity ATPase activity ATPase activity, coupled to transmembrane movement of substances

Process(es)

cholesterol biosynthetic process transport ion transport chloride transport water transport bicarbonate transport iodide transport cholesterol transport transepithelial chloride transport lung development vasodilation positive regulation of vasodilation sperm capacitation intracellular pH elevation transmembrane transport membrane hyperpolarization cellular response to cAMP chloride transmembrane transport positive regulation of voltage-gated chloride channel activity vesicle docking involved in exocytosis respiratory gaseous exchange positive regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of exocytosis positive regulation of cyclic nucleotide-gated ion channel activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CFTR Monoclonal Antibody (CF3)

CFTR Antibody (MA1-935) in ICC/IF

Product Details

MA1-935

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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