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CEBPE Monoclonal Antibody (7A4)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite CEBPE Monoclonal Antibody (7A4)

  • Antibody Testing Data (2)
CEBPE Antibody in Western Blot (WB)
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CEBPE Antibody in Western Blot (WB)
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CEBPE Antibody (H00001053-M01) in WB

Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Cat # H00001053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 (Cat # H00001053-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CEBPE Antibody in Western Blot (WB)
CEBPE Antibody in Western Blot (WB)
CEBPE Monoclonal Antibody (7A4)

Product Details

H00001053-M01

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

7A4

Immunogen

CEBPE (AAH35797.2, 1 a.a. approximately 281 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MSHGTYYECE PRGGQQPLEF SGGRAGPGEL GDMCEHEASI DLSAYIESGE EQLLSDLFAV KPAPEARGLK GPGTPAFPHY LPPDPRPFAY PPHTFGPDRK ALGPGIYSSP GSYDPRAVAV KEEPRGPEGS RAASRGSYNP LQYQVAHCGQ TAMHLPPTLA APGQPLRVLK APLATAAPPC SPLLKAPSPA GPLHKGKKAV NKDSLEYRLR RERNNIAVRK SRDKAKRRIL ETQQKVLEYM AENERLRSRV EQLTQELDTL RNLFRQIPEA ANLIKGVGGC S

Target Information

C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C EBP; C/EBP epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon

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Gene Aliases: C/EBP-epsilon; CEBPE; CRP1

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UniProt ID: (Human) Q15744

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Entrez Gene ID: (Human) 1053

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein homodimerization activity protein heterodimerization activity
Process(es)
transcription from RNA polymerase II promoter phagocytosis defense response macrophage differentiation cytokine biosynthetic process defense response to bacterium positive regulation of transcription from RNA polymerase II promoter cellular response to lipopolysaccharide
It has to be done as per old AB suggested Products section.
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