Invitrogen

CDK9 Monoclonal Antibody (K.513.1)

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2 Published Figures
4 References
View all (29) CDK9 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
CDK9 Monoclonal Antibody (K.513.1)

Product Details

MA5-14912

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-

Flow Cytometry (Flow)

1:100-1:400
-

Immunoprecipitation (IP)

1:100
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.513.1

Immunogen

Synthetic peptide corresponding to residues near the carboxy terminus of human CDK9
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

163 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986858

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

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Gene Aliases: BOS_12097; C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK

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UniProt ID: (Human) P50750, (Bovine) Q5EAB2, (Mouse) Q99J95, (Rat) Q641Z4

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Entrez Gene ID: (Human) 1025, (Bovine) 520580, (Dog) 100855805, (Chinese hamster) 100757719, (Mouse) 107951, (Rat) 362110

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Function(s)
RNA polymerase II core promoter sequence-specific DNA binding transcription coactivator binding DNA binding chromatin binding protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity 7SK snRNA binding snRNA binding transcription regulatory region DNA binding nucleotide binding transcription factor binding transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
DNA repair regulation of DNA repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter protein phosphorylation regulation of mitotic cell cycle cell proliferation positive regulation of cardiac muscle hypertrophy regulation of histone modification replication fork processing positive regulation of histone phosphorylation response to drug snRNA transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription regulation of muscle cell differentiation negative regulation of cell cycle arrest cellular response to cytokine stimulus negative regulation of mRNA polyadenylation positive regulation of mRNA 3'-UTR binding positive regulation of histone H2B ubiquitination transcription, DNA-templated regulation of transcription, DNA-templated replication fork arrest cellular response to DNA damage stimulus phosphorylation
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