Invitrogen

CDK4 Polyclonal Antibody

Name: CDK4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-14445

View all (63) CDK4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

CDK4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CDK4 Antibody (PA5-14445) in ICC/IF

Immunocytochemistry analysis of CDK4 in HeLa cells. Samples were incubated with CDK4 polyclonal antibody (Product # PA5-14445) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). CDK4 immunoreactivity is localized to Cytoplasm signif... View More

CDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-14445

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:10-1:50

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2291635

Target Information

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell division protein kinase 4; Cyclin-dependent kinase 4; MGC14458; PSK-J3

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Gene Aliases: CDK4; CMM3; PSK-J3

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UniProt ID: (Human) P11802

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Entrez Gene ID: (Human) 1019

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Function(s)

cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding cyclin-dependent protein serine/threonine kinase regulator activity cyclin binding protein complex binding

Process(es)

G1/S transition of mitotic cell cycle lens development in camera-type eye protein phosphorylation signal transduction circadian rhythm positive regulation of cell proliferation response to toxic substance response to lead ion regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle organ regeneration response to testosterone response to drug positive regulation of apoptotic process positive regulation of translation positive regulation of cell cycle positive regulation of cell size regulation of protein kinase activity positive regulation of fibroblast proliferation cell division response to hyperoxia negative regulation of cell cycle arrest

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CDK4 Polyclonal Antibody

CDK4 Antibody (PA5-14445) in ICC/IF

Product Details

PA5-14445

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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