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Invitrogen
CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD90 (Thy-1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 46
CD90 (Thy-1) Antibody (14-0909-82) in Flow
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD90 (Thy-1) Antibody (14-0909-82) in Flow")
Product Details
14-0909-82
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Product Specific Information
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.
Applications Tested: This eBio5E10 (5E10) antibody has been tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen
Gene Aliases: CD90; CDw90; THY1
UniProt ID: (Human) P04216
Entrez Gene ID: (Human) 7070
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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