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Invitrogen
CD223 (LAG-3) Monoclonal Antibody (3DS223H), Alexa Fluor™ 660, eBioscience™
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CD223 (LAG-3) Antibody (606-2239-42) in Flow
Product Details
606-2239-42
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Product Specific Information
Description: The 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
This 3DS223H antibody will recognize a formaldehyde-fixed epitope.
Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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References (0)
Bioinformatics
Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3
Gene Aliases: CD223; FDC; LAG3
UniProt ID: (Human) P18627
Entrez Gene ID: (Human) 3902
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