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Invitrogen
CD205 Monoclonal Antibody (205yekta), PE-eFluor™ 610, eBioscience™
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CD205 Antibody (61-2051-82) in Flow
Product Details
61-2051-82
Species Reactivity
Host/Isotype
Recommended Isotype Control
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Type
Clone
Conjugate
Excitation/Emission Max
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Purification
Storage buffer
Contains
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RRID
Product Specific Information
Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.
CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.
Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 205yekta antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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References (0)
Bioinformatics
Protein Aliases: CA-IX; CD205; DCL-1 fusion; DEC-205; gp200; Ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RP23-10L17.4
Gene Aliases: CD205; DEC-205; DEC205; Ly75
UniProt ID: (Mouse) Q60767
Entrez Gene ID: (Mouse) 17076
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