Invitrogen

CD205 Monoclonal Antibody (205yekta), PE-Cyanine7, eBioscience™

Name: CD205 Monoclonal Antibody (205yekta), PE-Cyanine7, eBioscience\u2122
Brand: Invitrogen
SKU: 25-2051-42

1 Published Figure

9 References

View all (38) CD205 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

CD205 Antibody (25-2051-42) in Flow

Staining of mouse splenocytes with Anti-Mouse CD11c FITC (Product # 11-0114-82), Anti-Mouse CD8a APC (Product # 17-0081-82) and Anti-Mouse CD205 PE-Cyanine7. The histogram (right) demonstrates staining of CD205 on CD11c+CD8a. cells (purple histogram) and CD11c+CD8a+ cells (blue histogram), as gated in the dot plot (left).

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Product Details

25-2051-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.007 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

C. elegans, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

205yekta

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1834473

Product Specific Information

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.

CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.

Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 205yekta antibody has been pre-titrated and tested by flow cytometric analysis of in vitro-cultured bone marrow-derived dendritic cells or mouse Granulocytes. This can be used at 5 µL (0.007 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity.

PLoS neglected tropical diseases

Bhattacharya P,Ismail N,Saxena A,Gannavaram S,Dey R,Oljuskin T,Akue A,Takeda K,Yu J,Karmakar S,Dagur PK,McCoy JP,Nakhasi HL

25-2051-42 was used in Flow Cytometry to show that LdCen-/- containing neutrophils phagocytized by DC markedly influence the phenotype and antigen presenting capacity of DCs early on and thus play an immune-regulatory role in shaping vaccine induced host protective response.

Tue Feb 01 00:00:00 UTC 2022

Alveolar macrophages instruct CD8⁺ T cell expansion by antigen cross-presentation in lung.

Cell reports

Kawasaki T,Ikegawa M,Yunoki K,Otani H,Ori D,Ishii KJ,Kuroda E,Takamura S,Kitabatake M,Ito T,Isotani A,Kawai T

25-2051 was used in Flow cytometry/Cell sorting to suggest that AMs instruct a rapid expansion of antigen-specific CD8+ T cells in lung, which protect the host from respiratory virus infection.

Tue Dec 13 00:00:00 UTC 2022

Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.

The EMBO journal

Kawasaki T,Ito K,Miyata H,Akira S,Kawai T

Published figure using CD205 monoclonal antibody (Product # 25-2051-42) in Flow Cytometry

Wed Jun 14 00:00:00 UTC 2017

Geldanamycin Reduces Acute Respiratory Distress Syndrome and Promotes the Survival of Mice Infected with the Highly Virulent H5N1 Influenza Virus.

Frontiers in cellular and infection microbiology

Wang C,Liu P,Luo J,Ding H,Gao Y,Sun L,Luo F,Liu X,He H

25-2051 was used in Flow cytometry/Cell sorting to show geldanamycin plays an important role in attenuating virus infection-induced acute lung injury/acute respiratory distress syndrome by reducing the host's inflammatory responses.

Mon Feb 26 00:00:00 UTC 2018

MIF Promotes Classical Activation and Conversion of Inflammatory Ly6C(high) Monocytes into TipDCs during Murine Toxoplasmosis.

Mediators of inflammation

Ruiz-Rosado Jde D,Olguín JE,Juárez-Avelar I,Saavedra R,Terrazas LI,Robledo-Avila FH,Vazquez-Mendoza A,Fernández J,Satoskar AR,Partida-Sánchez S,Rodriguez-Sosa M

25-2051 was used in Flow cytometry/Cell sorting to analyse the role of migration inhibitory factor in the maturation of CD11b(+) and CD8α (+) dendritic cells.

Fri Dec 30 00:00:00 UTC 2016

Ultraviolet B light attenuates the systemic immune response in central nervous system autoimmunity.

Annals of neurology

Breuer J,Schwab N,Schneider-Hohendorf T,Marziniak M,Mohan H,Bhatia U,Gross CC,Clausen BE,Weishaupt C,Luger TA,Meuth SG,Loser K,Wiendl H

25-2051 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the beneficial effects of immune regulatory processes induced in the skin by moderate ultraviolet B (UVB) radiation on central nervous system (CNS) autoimmunity.

Thu May 01 00:00:00 UTC 2014

Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors.

European journal of immunology

Baik S,Jenkinson EJ,Lane PJ,Anderson G,Jenkinson WE

25-2051 was used in Flow cytometry/Cell sorting to reveal an unexpected overlap in the phenotypic properties of bipotent TECs with their lineage-restricted counterparts.

Fri Mar 01 00:00:00 UTC 2013

Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection.

Cell

Teijaro JR,Walsh KB,Cahalan S,Fremgen DM,Roberts E,Scott F,Martinborough E,Peach R,Oldstone MB,Rosen H

25-2051 was used in Flow cytometry/Cell sorting to elucidate cellular and signalling mechanisms that are important in initiating cytokine storm.

Fri Sep 16 00:00:00 UTC 2011

UV exposure boosts transcutaneous immunization and improves tumor immunity: cytotoxic T-cell priming through the skin.

The Journal of investigative dermatology

Stein P,Rechtsteiner G,Warger T,Bopp T,Fuhr T,Prüfer S,Probst HC,Stassen M,Langguth P,Schild H,Radsak MP

25-2051 was used in Flow Cytometry to explore the effects of ultraviolet irradiation on imiquimod-based transcutaneous immunisation.

Sat Jan 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CA-IX; CD205; DCL-1 fusion; DEC-205; gp200; Ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RP23-10L17.4

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Gene Aliases: CD205; DEC-205; DEC205; Ly75

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UniProt ID: (Mouse) Q60767

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Entrez Gene ID: (Mouse) 17076

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Function(s)

carbohydrate binding

Process(es)

endocytosis

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CD205 Monoclonal Antibody (205yekta), PE-Cyanine7, eBioscience™

CD205 Antibody (25-2051-42) in Flow

Product Details

25-2051-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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