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OriGene

CAD Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite CAD Polyclonal Antibody

  • Antibody Testing Data (2)
CAD Antibody in Western Blot (WB)
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CAD Antibody in Western Blot (WB)
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CAD Antibody (TA890104) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAD (RC209469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CAD rabbit polyclonal antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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CAD Antibody in Western Blot (WB)
CAD Antibody in Western Blot (WB)

Product Details

TA890104

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein of human CAD

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.91 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CAD trifunctional protein; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; Multifunctional protein CAD

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Gene Aliases: CAD; CDG1Z

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UniProt ID: (Human) P27708

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Entrez Gene ID: (Human) 790

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Function(s)
aspartate carbamoyltransferase activity carbamoyl-phosphate synthase (ammonia) activity carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity dihydroorotase activity protein kinase activity ATP binding zinc ion binding enzyme binding identical protein binding aspartate binding
Process(es)
urea cycle liver development 'de novo' pyrimidine nucleobase biosynthetic process UTP biosynthetic process arginine biosynthetic process glutamine metabolic process heart development female pregnancy lactation response to amine drug metabolic process peptidyl-threonine phosphorylation response to caffeine organ regeneration response to testosterone cellular response to drug 'de novo' UMP biosynthetic process pyrimidine nucleoside biosynthetic process protein autophosphorylation response to cortisol cellular response to epidermal growth factor stimulus
It has to be done as per old AB suggested Products section.
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