Invitrogen

CAD Polyclonal Antibody

Name: CAD Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78903

View all (22) CAD antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

CAD Antibody (PA5-78903) in WB

Western blot analysis of CAD in Lane 1: mouse pancreas tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CAD polyclonal antibody (Product # PA5-78903) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary ant... View More

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Immunogen image

Immunogen Image CAD Polyclonal Antibody

Product Details

PA5-78903

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

ELISA (ELISA)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human CAD recombinant protein (Position: L1555-K1780).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2746019

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: mouse pancreas tissue.

Target Information

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CAD protein; CAD trifunctional protein; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; Multifunctional protein CAD

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Gene Aliases: 2410008J01Rik; AU018859; CAD; CDG1Z; Cpad

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UniProt ID: (Human) P27708, (Mouse) B2RQC6

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Entrez Gene ID: (Human) 790, (Mouse) 69719

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Function(s)

aspartate carbamoyltransferase activity carbamoyl-phosphate synthase (ammonia) activity carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity dihydroorotase activity protein kinase activity ATP binding zinc ion binding enzyme binding identical protein binding aspartate binding nucleotide binding UTP binding catalytic activity amino acid binding transferase activity carboxyl- or carbamoyltransferase activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides ligase activity metal ion binding

Process(es)

urea cycle 'de novo' pyrimidine nucleobase biosynthetic process pyrimidine nucleotide biosynthetic process UTP biosynthetic process cellular amino acid metabolic process arginine biosynthetic process glutamine metabolic process nitrogen compound metabolic process metabolic process drug metabolic process peptidyl-threonine phosphorylation protein autophosphorylation liver development heart development female pregnancy lactation response to amine response to caffeine organ regeneration response to testosterone cellular response to drug 'de novo' UMP biosynthetic process pyrimidine nucleoside biosynthetic process response to cortisol cellular response to epidermal growth factor stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CAD Polyclonal Antibody

CAD Antibody (PA5-78903) in WB

Product Details

PA5-78903

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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